Volume 21, Issue 1 (Spring 2024)
Sci J Iran Blood Transfus Organ 2024, 21(1): 33-45 |
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Shafiei dar Afshani M, Shahanipour K, Shamsfar R, Jamshidian H. Investigating the effect of antioxidant compounds of L-carnitine, Resveratrol, and Vitamin C during the storage time of platelet concentrate. Sci J Iran Blood Transfus Organ 2024; 21 (1) :33-45
URL: http://bloodjournal.ir/article-1-1514-en.html
URL: http://bloodjournal.ir/article-1-1514-en.html
Abstract: (682 Views)
Abstract
Background and Objectives
The storage time of platelet concentrate is limited to 3-5 days due to various factors, including bacterial contamination and platelet storage lesions. Antioxidant agents have been found to significantly improve the survival and function of platelets. The purpose of this study was to investigate the effect of the antioxidant compounds L-carnitine, Resveratrol, and Vitamin C on platelet oxidative stress during storage.
Materials and Methods
This experimental study utilized six platelet concentrate bags to evaluate the antioxidant effect of L-carnitine, Resveratrol, and Vitamin C at concentrations of 50 and 100 mM. The effects of these antioxidants on malondialdehyde (MDA) levels, lactate dehydrogenase (LDH) enzyme activity, H2O2, and pH levels were measured on days 0, 3, and 5 of platelet storage. The data was analyzed using GraphPad Prism version 8 software and one-way ANOVA tests.
Results
The concentration of 100 mM antioxidant compounds had a stronger protective effect compared to 50 mM. These effects were significant on the fifth day of platelet storage. The impact of these antioxidants resulted in improving platelet quality, including a decrease in MDA levels, and LDH activity.
Conclusions
The results showed that the antioxidant compounds can affect oxidative conditions created during five days of storage and reduce platelet destruction during this time through a specific biochemical mechanism.
Background and Objectives
The storage time of platelet concentrate is limited to 3-5 days due to various factors, including bacterial contamination and platelet storage lesions. Antioxidant agents have been found to significantly improve the survival and function of platelets. The purpose of this study was to investigate the effect of the antioxidant compounds L-carnitine, Resveratrol, and Vitamin C on platelet oxidative stress during storage.
Materials and Methods
This experimental study utilized six platelet concentrate bags to evaluate the antioxidant effect of L-carnitine, Resveratrol, and Vitamin C at concentrations of 50 and 100 mM. The effects of these antioxidants on malondialdehyde (MDA) levels, lactate dehydrogenase (LDH) enzyme activity, H2O2, and pH levels were measured on days 0, 3, and 5 of platelet storage. The data was analyzed using GraphPad Prism version 8 software and one-way ANOVA tests.
Results
The concentration of 100 mM antioxidant compounds had a stronger protective effect compared to 50 mM. These effects were significant on the fifth day of platelet storage. The impact of these antioxidants resulted in improving platelet quality, including a decrease in MDA levels, and LDH activity.
Conclusions
The results showed that the antioxidant compounds can affect oxidative conditions created during five days of storage and reduce platelet destruction during this time through a specific biochemical mechanism.
Keywords: Key words: Blood Platelets, Antioxidants, Malondialdehyde, L-Lactate Dehydrogenase, Hydrogen Peroxide
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