Volume 13, Issue 2 (Summer 2016)                   Sci J Iran Blood Transfus Organ 2016, 13(2): 130-137 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Razavi Babaheydari S, Mousavi Hosseini K, Atashi A, Esmaeeili S. Study of quantitative and qualitative gene expression of lipoprotein lipase before and after differentiation of human bone marrow mesenchymal stem cells into adipocytes. Sci J Iran Blood Transfus Organ 2016; 13 (2) :130-137
URL: http://bloodjournal.ir/article-1-993-en.html
Study of quantitative and qualitative gene expression of lipoprotein lipase before and after differentiation of human bone marrow mesenchymal stem cells into adipocytes
S.R. Razavi Babaheydari , K. Mousavi Hosseini * , A. Atashi , Sh. Esmaeeili
Keywords: Key words: Mesenchymal Stromal Cells, Adipocytes, Lipoprotein Lipase, Cell Differentiation
Full-Text [PDF 468 kb]   (1382 Downloads)     |   Abstract (HTML)  (5746 Views)
Full-Text:   (1798 Views)
References :  
  1. Whitelaw NC, Whitelaw E. Transgenerational epigenetic inheritance in health and disease. Curr Opin Genet Dev 2008; 18(3): 273-9.
  2. Pastinen T, Sladek R, Gurd S, Sammak A, Ge B, Lepage P, et al. A survey of genetic and epigenetic variation affecting human gene expression. Physiol Genomics 2004; 16(2): 184-93.
  3. Vaissiere T, Sawan C, Herceg Z. Epigenetic interplay between histone modifications and DNA methylation in gene silencing. Mutat Res 2008; 659(1-2): 40-8.
 
 
 
  1. ubicek S, Schotta G, Lachner M, Sengupta R, Kohlmaier A, Perez-Burgos L, et al. The role of histone modifications in epigenetic transitions during normal and perturbed development. Ernst Schering Res Found Workshop 2006; (57): 1-27.
  2. Mohtat D, Susztak K. Fine tuning gene expression: the epigenome. Semin Nephrol 2010; 30(5): 468-76.
  3. Bohacek J, Mansuy IM. Epigenetic inheritance of disease and disease risk. Neuropsychopharmacology 2013; 38(1): 220-36.
  4. Lund AH,van Lohuizen M. Epigenetics and cancer. Genes Dev 2004; 18(19): 2315-35.
  5. Berdasco M,Esteller M. Hot topics in epigenetic mechanisms of aging. Aging Cell 2012; 11(2): 181-6.
  6. Surani MA. Genomic imprinting: control of gene expression by epigenetic inheritance. Curr Opin Cell Biol 1994; 6(3): 390-5.
  7. Zhang YB, Chu JY. Progress of research on epigenetic and human disease. Yi Chuan 2005; 27(3): 466-72. [Article in Chinese]
  8. Turner BM. Histone acetylation and an epigenetic code. Bioessays 2000; 22(9): 836-45.
  9. Chen S, Bohrer LR, Rai AN, Pan Y, Gan L, Zhou X, et al. Cyclin-dependent kinases regulate epigenetic gene silencing through phosphorylation of EZH2. Nat Cell Biol 2010; 12(11): 1108-14.
  10. Calvo V, Beato M. BRCA1 counteracts progesterone action by ubiquitination leading to progesterone receptor degradation and epigenetic silencing of target promoters. Cancer Res 2011; 71(9): 3422-31.
  11. Stielow B,  Krüger I, Diezko R, Finkernagel F, Gillemans N, Kong-a-San J, et al. Epigenetic silencing of spermatocyte-specific and neuronal genes by SUMO modification of the transcription factor Sp3. PLoS Genet 2010; 6(11): e1001203.
  12. Klose RJ, Bird AP. Genomic DNA methylation: the mark and its mediators. Trends Biochem Sci 2006; 31(2): 89-97.
  13. Bird AP. Functions for DNA methylation in vertebrates. Cold Spring Harb Symp Quant Biol 1993; 58: 281-5.
  14. Guz J, Foksinski M, Olinski R. Mechanism of DNA methylation and demethylation--its role in control of genes expression. Postepy Biochem 2010; 56(1): 7-15. [Article in Polish]
  15. Hendrich B, Bird A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol 1998; 18(11): 6538-47.
  16. Lan J, Hua S, He X, Zhang Y. DNA methyltransferases and methyl-binding proteins of mammals. Acta Biochim Biophys Sin (Shanghai) 2010; 42(4): 243-52.
 
  1. Feinberg AP, Cui H, Ohlsson R. DNA methylation and genomic imprinting: insights from cancer into epigenetic mechanisms. Semin Cancer Biol 2002; 12(5): 389-98.
  2. Rottach A, Leonhardt H, Spada F. DNA methylation-mediated epigenetic control. J Cell Biochem 2009; 108(1): 43-51.
  3. Byvoet P, Sayre DF, Baxter CS. Methods for the assessment of histone methylation. Methods Cell Biol 1978; 19: 95-100.
  4. Mack FA, Patel JH, Biju MP, Haase VH, Simon MC. Decreased growth of Vhl-/- fibrosarcomas is associated with elevated levels of cyclin kinase inhibitors p21 and p27. Mol Cell Biol 2005; 25(11): 4565-78.
  5. Tollefsbol TO. Methods of epigenetic analysis. Methods Mol Biol 2004; 287: 1-8.
  6. Otera H, Harano T, Honsho M, Ghaedi K, Mukai S, Tanaka A, et al. The Mammalian Peroxin Pex5pL,the Longer Isoform ofthe Mobile Peroxisome Targeting Signal (PTS) Type 1Transporter,Translocates the Pex7pzPTS2 ProteinComplex into Peroxisomes via Its Initial Docking Site,Pex14p. J Biol Chem 2000; 275(28): 21703-14.
  7. Ghaedi K, Kawai A, Okumoto K, Tamura S, Shimozawa N, Suzuki Y, et al Isolation and characterization of novel peroxisome biogenesis-defective Chinese hamster ovary cell mutants using green fluorescent protein. Exp Cell Res 1999; 248(2): 489-97.
  8. Fujiki Y, Okumoto K, Kinoshita N, Ghaedi K. Lessons from peroxisome-deficient Chinesehamster ovary (CHO) cell mutants. Biochim Biophys Acta 2006; 1763(12): 1374-81.
  9. Ghaedi K, Masanori H, Shimozawa N, Suzuki Y, Kondo N, Yukio F. PEX3 Is the causal generesponsible for peroxisome membrane assembly-defectivezellweger syndrome of complementation group G. Am J Hum Genet 2000; 67(4): 976-81.
  10. Hihi AK, Michalik L, Wahli W. PPARs :transcriptional effectors of fatty acids and their derivatives. Cell Mol Life Sci 2002; 59(5): 790-8.
 
 
 
 
 
 
 

 
Original  Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2016; 13(2): 130-137
 
 

Study of quantitative and qualitative gene expression of lipoprotein lipase before and after differentiation of human bone marrow mesenchymal stem cells into adipocytes
Razavi Babaheidary S.R.1, Mousavi Hosseini K.1, Atashi A.2, Esmaeeili Sh.2
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
 
 
Abstract
Background and Objectives
Serum TG plays an important role in carcinogenesis and the LPL as a key enzyme that hydrolyses plasma TG involves in this process. Abnormal regulation and/or expression of LPL play roles in many human diseases such as atherosclerosis. Serum TG levels increase the risk of chylomicronaemia, obesity and type 2 diabetes; thus, in this study we reported LPL expression before and after BM-MSCs differentiation into adipocyte under controlled conditions.
                                                                                                                                                                                                   
Materials and Methods
MSCs from human bone marrow were isolated from 3 healthy people and then differentiated into adipocytes by chemical materials for 14 days. Then, total RNA was extracted and cDNA was synthesized and finally RT-PCR and Real Time PCR analysis were performed for LPL gene.
 
Results
After 14 days, the analysis of morphology with invert microscopy indicated that BM-MSCs have the capacity of differentiation into adipocytes. BM-MSCs serving as the negative control do not have any expression of LPL while in BM-MSCs derived-adipocytes there has been the significant expression of LPL.
 
Conclusions
BM-MSCs can differentiate into either adipocytes or osteoblastic cells; LPL has the important role in the differentiation decision for either one. The inhibition or stimulation of LPL expression can be used for the treatment of various diseases including prostate cancer, aplastic anemia, and osteoporosis.
 
Key words: Mesenchymal Stromal Cells, Adipocytes, Lipoprotein Lipase, Cell Differentiation
 
 
 
Received:  24 Sep 2015
Accepted: 30 Jan 2016
 
 

Correspondence: Mousavi Hosseini K., PhD in Medicinal Chemistry. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052160; Fax: (+9821) 88601599
E-mail:
mkmousavi@yahoo.com
Correspondence: Atashi A., PhD of Hematology & Blood Banking. Assistant Professor of Faculty of Medical Sciences, Tarbiat Modares University.
Postal Code: 14115-111, Tehran, Iran. Tel: (+9821) 82883579; Fax: (+9821) 82884507
E-mail:
atashia@modares.ac.ir
Type of Study: Research | Subject: Stem cells
Published: 2016/06/15
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Scientific Journal of Iran Blood Transfus Organ

Designed & Developed by : Yektaweb