Study of quantitative and qualitative gene expression of lipoprotein lipase before and after differentiation of human bone marrow mesenchymal stem cells into adipocytes

Razavi Babaheidary S.R.¹, Mousavi Hosseini K.¹, Atashi A.², Esmaeeili Sh.²

¹Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

²School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

Background and Objectives

Serum TG plays an important role in carcinogenesis and the LPL as a key enzyme that hydrolyses plasma TG involves in this process. Abnormal regulation and/or expression of LPL play roles in many human diseases such as atherosclerosis. Serum TG levels increase the risk of chylomicronaemia, obesity and type 2 diabetes; thus, in this study we reported LPL expression before and after BM-MSCs differentiation into adipocyte under controlled conditions.

Materials and Methods

MSCs from human bone marrow were isolated from 3 healthy people and then differentiated into adipocytes by chemical materials for 14 days. Then, total RNA was extracted and cDNA was synthesized and finally RT-PCR and Real Time PCR analysis were performed for LPL gene.

Results

After 14 days, the analysis of morphology with invert microscopy indicated that BM-MSCs have the capacity of differentiation into adipocytes. BM-MSCs serving as the negative control do not have any expression of LPL while in BM-MSCs derived-adipocytes there has been the significant expression of LPL.

Conclusions

BM-MSCs can differentiate into either adipocytes or osteoblastic cells; LPL has the important role in the differentiation decision for either one. The inhibition or stimulation of LPL expression can be used for the treatment of various diseases including prostate cancer, aplastic anemia, and osteoporosis.