Volume 11, Issue 4 (winter 2015)
Sci J Iran Blood Transfus Organ 2015, 11(4): 281-285 |
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Omidkhoda A, Nadali F, Babadivadn P, Sahlolbeii M, Amini Kafiabad S. The effect of the delayed interval between the separation of plasma and freezing on FVIII activity in FFP . Sci J Iran Blood Transfus Organ 2015; 11 (4) :281-285
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URL: http://bloodjournal.ir/article-1-839-en.html
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| Abstract (HTML) (7931 Views)
The effect of the delayed interval between
the separation of plasma and freezing on FVIII
activity in FFP
Omidkhoda A.1,2, Nadali F.1,2,3, Babadivand P.1,2, Sahlolbeii M.1,2, Amini Kafi-Abad S.1
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Tehran Regional Educational Blood Transfusion Center, Tehran, Iran
3School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Background and Objectives
Plasma is an important product for the preparation of coagulation factor concentrates. FVIII is one of the labile coagulation factors in plasma and its activity is used as a quality marker of plasma; therefore, it is important to optimize plasma production in order to avoid a reduction of FVIII activity. In this study, the effect of the delayed interval from plasma production to freezing on factor VIII activity in FFP in Iran was evaluated.
Materials and Methods
Thirty whole blood units in pediatric bags were collected and their plasma aliquotes were separated. The plasma was then divided into three parts equally. The first, second, and third parts were frozen within 1 hour, 2 hours and 6 hours of separation, respectively. FVIII activity was then measured by the one-stage clotting assays.
Results
The average rates of factor VIII activity in the first, second, and third groups were 1.78 ± 0.4 IU/mL, 1.72 ± 0.4 IU/mL, and 1.5 ± 0.3 IU/mL, respectively
Conclusions
To provide fresh frozen plasma, it is necessary to freeze plasma within 2 hours after whole blood separation. Although any increase in this time to 6 hours is associated with the significant decline in factor VIII activity compared to 1 and 2 hours, it remains within the acceptable level.
Key words: Fresh Frozen Plasma, Time, Factor VIII, Freezing
Received: 14 Mar 2014
Accepted: 14 Jun 2014
Correspondence: Amini Kafi-Abad S, MD. Specialist in Pathology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601558; Fax: (+9821) 88601542
E-mail: s.amini@ibto.ir
Full-Text: (3320 Views)
References:
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- Omidkhoda A, Tabatabaei MR, Atarodi K, Karimi K, Rahimi Froushani A, Pourfathollah AA. A comparative study of the effects of temperature, time and factor VIII assay type on factor VIII activity in cryoprecipitate in Iran. Blood Transfuse 2011; 9(4): 394-9.
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factor VIII:C concentrates. Storage of whole blood and plasma and interindividual plasma levels of fibrinopeptide A. Vox Sang 1983; 45(3): 233-42. - What are the critical factors in the production and quality control of frozen plasma intended for direct transfusion or for fractionation to provide medically needed labile coagulation factors? Vox Sang 1983; 44(4): 246-59.
- Carlebjörk G, Blombäck M, Akerblom O. Improvement of plasma quality as raw material for factor VIII: C concentrate. Storage of whole blood and plasma and inter individual plasma levels of fibrinopeptide A. Vox Sang 1983; 45(3): 233-42.
- Smith JF, Ness PM, Moroff G, Luban NL. Retention of coagulation factors in plasma frozen after extended holding at 1-6 degrees C. Vox Sang 2000; 78(1): 28-30
- Cardigan R, Lawrie AS, Mackie IJ, Williamson LM. The quality of fresh-frozen plasma produced from whole blood stored at 4 degrees C overnight. Transfusion 2005; 45(8): 1342-8.
- Serrano K, Scammell K, Weiss S, Culibrk B, Levin E, Gyöngyössy-Issa M, et al. Plasma and cryoprecipitate manufactured from whole blood held overnight at room temperature meet quality standards. Transfusion 2010; 50(2): 344-53.
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Sci J Iran Blood Transfus Organ 2015; 11(4): 281-285 |
|
Original Article
|
The effect of the delayed interval between
the separation of plasma and freezing on FVIII
activity in FFP
Omidkhoda A.1,2, Nadali F.1,2,3, Babadivand P.1,2, Sahlolbeii M.1,2, Amini Kafi-Abad S.1
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Tehran Regional Educational Blood Transfusion Center, Tehran, Iran
3School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Background and Objectives
Plasma is an important product for the preparation of coagulation factor concentrates. FVIII is one of the labile coagulation factors in plasma and its activity is used as a quality marker of plasma; therefore, it is important to optimize plasma production in order to avoid a reduction of FVIII activity. In this study, the effect of the delayed interval from plasma production to freezing on factor VIII activity in FFP in Iran was evaluated.
Materials and Methods
Thirty whole blood units in pediatric bags were collected and their plasma aliquotes were separated. The plasma was then divided into three parts equally. The first, second, and third parts were frozen within 1 hour, 2 hours and 6 hours of separation, respectively. FVIII activity was then measured by the one-stage clotting assays.
Results
The average rates of factor VIII activity in the first, second, and third groups were 1.78 ± 0.4 IU/mL, 1.72 ± 0.4 IU/mL, and 1.5 ± 0.3 IU/mL, respectively
Conclusions
To provide fresh frozen plasma, it is necessary to freeze plasma within 2 hours after whole blood separation. Although any increase in this time to 6 hours is associated with the significant decline in factor VIII activity compared to 1 and 2 hours, it remains within the acceptable level.
Key words: Fresh Frozen Plasma, Time, Factor VIII, Freezing
Received: 14 Mar 2014
Accepted: 14 Jun 2014
Correspondence: Amini Kafi-Abad S, MD. Specialist in Pathology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601558; Fax: (+9821) 88601542
E-mail: s.amini@ibto.ir
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