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Ashouri kafshgar E, Chegini A, Samiee S, Zadsar M. The Evaluation of Quality Parameters of Platelet Apheresis Products Obtained from the Fresenius COM.TEC Device during Storage. bloodj 2025; 22 (2) :150-159
URL: http://bloodjournal.ir/article-1-1563-en.html
The Evaluation of Quality Parameters of Platelet Apheresis Products Obtained from the Fresenius COM.TEC Device during Storage
Elahe Ashouri kafshgar , Azita Chegini * , Shahram Samiee , Maryam Zadsar
Keywords: Plateletpheresis, Quality control, Blood Platelets, Platelet Transfusion
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   References:
  1. Rinder HM, Tomer A. Platelet production, kinetics , and hemostasis. In: Simon TL, Snyder EL, Solheim BG, Stowell ChP, Strauss RG, Petrides M. Rossi's Principles of Transfusion Medicine. 4th ed. USA: Blackwell Publishing; 2009. p. 149-67. [DOI:10.1002/9781444303513.ch10]
  2. Rinder HM, Tomer A. Platelet production, kinetics, and hemostasis. Rossi's Principles of Transfusion Medicine. 2009 Jan 9:149-67. [DOI:10.1002/9781444303513.ch10]
  3. Ribatti D, Crivellato E. Giulio Bizzozero and the discovery of platelets. Leuk Res 2007; 31(10): 1339-41. [DOI:10.1016/j.leukres.2007.02.008] [PMID]
  4. Javadzadeh Shahshahani H, Akhavan Tafti F, Amini Kafi‐abad S. An overview of three methods used to prepare the platelet components from whole blood and apheresis method. Sci J Iran Blood Transfus Organ 2023; 20(3): 236-54. [Article in Farsi]
  5. Hillyer CD, Silberstein LE, Ness PM, Anderson KC, Roback JD. Blood Banking and Transfusion Medicine: Basic Principle and Practice. 2nd ed. USA: Churchill Livingstone; 2007. p.735-6.
  6. McLeod BC, Weinstein R, Winters JL, Szczepiorkowski ZM. Apheresis: Principles and Practice. 3rd ed. USA: AABB; 2010. p. 2-23.
  7. Smith J, Burgstaler E. Blood component collection by apheresis. In: Roback JD, Combs MR, Grossman BJ, Hillyer ChD. Technical Manual. 17th ed. USA: AABB; 2008. p. 227-38.
  8. Chegini A, Mirrezaie M, Poorreza M, Rezaie A, Anbardan A. Assessment of plateletphersis donation in Tehran Blood Center. Sci J Iran Blood Transfus Organ 2013; 10(2): 140-6. [Article in Farsi]
  9. Sadeghi Neysiyani S , Amini-Kafiabad S. The effect of different types of plateletpheresis devices on the quality parameters of the produced platelet units. Sci J Iran Blood Transfus Organ 2024; 21(2): 169-84. [Article in Farsi]
  10. Singh RP, Marwaha N, Malhotra P, Dash S. Quality assessment of platelet concentrates prepared by platelet rich plasma-platelet concentrate, buffy coat poor-platelet concentrate (BC-PC) and apheresis-PC methods. Asian J Transfus Sci 2009; 3(2): 86-94 [DOI:10.4103/0973-6247.53882] [PMID] []
  11. Bertolini F, Rebulla P, Riccardi D, Cortellaro M, Ranzi ML, Sirchia G. Evaluation of platelet concentrates prepared from buffy coats and stored in a glucose‐free crystalloid medium. Transfusion 1989; 29(7): 605-9. [DOI:10.1046/j.1537-2995.1989.29789369678.x] [PMID]
  12. Sadeghi Neysiyani S, Amini Kafi-Abad S. The effect of different types of plateletpheresis devices on the quality parameters of the produced platelet units. Sci J Iran Blood Transfus Organ 2024; 21(2): 169-84. [Article in Farsi]
  13. Procházková R, Andrýs C, Hubáčková L, Krejsek J. Markers of platelet activation and apoptosis in platelet concentrates collected by apheresis. Transfus Apher Sci 2007; 37(2): 115-23. 
  15. [DOI:10.1016/j.transci.2007.03.015] [PMID]
  16. Macher S, Sipurzynski‐Budrass S, Rosskopf K, Rohde E, Griesbacher A, Groselj‐Strele A, et al. Function and activation state of platelets in vitro depend on apheresis modality. Vox Sang 2010; 99(4): 332-40. [DOI:10.1111/j.1423-0410.2010.01353.x] [PMID]
  17. Xia Y, Li J, Bertino A, Kuter D. Thrombopoietin and the TPO receptorduring platelet storage. Transfusion 2000; 40(8): 976-87. [DOI:10.1046/j.1537-2995.2000.40080976.x] [PMID]
  18. Ghasemi M, Turnbull T, Sebastian S, Kempson I. The MTT assay: utility, limitations, pitfalls, and interpretation in bulk and single-cell analysis. Int J Mol Sci 2021; 22(23): 12827. [DOI:10.3390/ijms222312827] [PMID] []
  19. Hussein   E.   Clinical  and  quality  evaluation  of
    apheresis  vs    random‐donor platelet concentrates stored for 7 days. Transfus Med 2015; 25(1): 20-6. [    DOI:10.1111/tme.12187] [PMID]
  20. Tynngård N, Lindahl TL, Trinks M, Studer M, Berlin G. The quality of platelet concentrates produced by COBE Spectra and Trima Accel cell separators during storage for 7 days as assessed by in vitro methods. Transfusion 2008; 48(4): 715-22. [DOI:10.1111/j.1537-2995.2007.01610.x] [PMID]
  21. Toora E, Kulkarni RG, Manivannan P, Sastry AS, Basavarajegowda A, Sahoo D. Quality assessment of platelet concentrates prepared by platelet-rich plasma, buffy-coat, and apheresis methods in a tertiary care hospital in South India: A cross-sectional study. Asian J Transfus Sci 2023; 17(2): 239-45. [DOI:10.4103/ajts.ajts_73_22] [PMID] []





The Evaluation of Quality Parameters of Platelet Apheresis Products Obtained from the Fresenius COM.TEC Device
during Storage


Elahe Ashouri Kafshgar1, Azita Chegini1        , Shahram Samiee1      , Maryam Zadsar1


1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

Received: 2024/11/12
Accepted: 2025/07/29
 





     http://dx.doi.org/10.61186/bloodj.21.4.296

 


   
Citation:
Ashouri kafshgar E, Chegini A, samiee Sh, Zadsar M. The Evaluation of Quality Parameters of Platelet Apheresis Products Obtained from the Fresenius COM.TEC Device during Storage. J Iran Blood Transfuse. 2025: 22 (2): 150-159
    



Correspondence: Chegini A., Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran.
Tel: (+9821) 82052256
E-mail: a.chegini@
1- Acridine Orange
1- Biological safety cabinet
1- Platelet Concentrate
2- Food and Drug Administration
3- Normal Skin Flora
4- Platelet Rich Plasma-Platelet Concentrate
5- Eosin-Methylene blue
6- Thioglycolate
1- Acridine Orange
1- Biological safety cabinet
1- Platelet Concentrate
2- Food and Drug Administration
3- Normal Skin Flora
4- Platelet Rich Plasma-Platelet Concentrate
5- Eosin-Methylene blue
6- Thioglycolate
tmi.ac.ir  		 A B S T R A C T
Background and Objectives
Platelets are small, nucleus-free cells vital for a variety of therapeutic interveutions. Ensuring the quality of transfused platelets is critical for optimal patient outcomes. Various devices are for separating platelet products from whole blood, each with unique characteristics. This study aimed to evaluate the Fresenius COM.TEC platelet apheresis machine by analyzing changes in pH, platelet count (PLT), Lactate dehydrogenase (LDH) levels, and platelet viability (MTT assay) across storage days 1, 3, and 5.
Materials and Methods
In this experimental study, 25 randomly selected platelet apheresis units from male donors were collected at the Tehran Blood Transfusion Center using the Fresenius COM.TEC device. The platelet products were stored at 20-24 °C in a shaker incubator. Samples were collected on storage days 1, 3, and 5 for evaluation of pH, LDH, PLT count, and platelet viability (MTT). All tests were conducted at the Blood Transfusion Research Center laboratory. Data analysis was performed using R software, and results with a p-values 0.05 were considered statistically significant.
Results
LDH levels were highest on day 5 and showed a significant increase throughout the storage period, with statistically significant differences between storage days. A consistent pH decline was also observed over time. MTT assay results indicated an transient increase on day 3, followed by a decline on day 5, with reaching statistical significance (p< 0.0001). in contrasts, platelet count did not exhibit any significant changes across all storage days (p> 0.05).
Conclusions 
There was no significant change in Platelet counts in apheresis products collected via the Fresenius COM.TEC device during storage. However, the observed pH decrease and LDH increase on days 1, 3, and 5 are consistent with trends reproted in similar studies. Interpretation of MTT assay outcomes should be approaches with caution due to its limitations, underscoring the need for robust control.
Key words: Plateletpheresis, Quality control, Blood Platelets, Platelet Transfusion
Copyright © 2025 Journal of Iranian Blood Transfusion, Published by Blood Transfusion Research Center.
This work is licensed under a Creative Common Attribution-Non Commercial 4.0 International license.



 
Type of Study: Research | Subject: Biotechnology
References
1. Rinder HM, Tomer A. Platelet production, kinetics , and hemostasis. In: Simon TL, Snyder EL, Solheim BG, Stowell ChP, Strauss RG, Petrides M. Rossi's Principles of Transfusion Medicine. 4th ed. USA: Blackwell Publishing; 2009. p. 149-67. [DOI:10.1002/9781444303513.ch10]
2. Rinder HM, Tomer A. Platelet production, kinetics, and hemostasis. Rossi's Principles of Transfusion Medicine. 2009 Jan 9:149-67. [DOI:10.1002/9781444303513.ch10]
3. Ribatti D, Crivellato E. Giulio Bizzozero and the discovery of platelets. Leuk Res 2007; 31(10): 1339-41. [DOI:10.1016/j.leukres.2007.02.008] [PMID]
4. Javadzadeh Shahshahani H, Akhavan Tafti F, Amini Kafi‐abad S. An overview of three methods used to prepare the platelet components from whole blood and apheresis method. Sci J Iran Blood Transfus Organ 2023; 20(3): 236-54. [Article in Farsi]
5. Hillyer CD, Silberstein LE, Ness PM, Anderson KC, Roback JD. Blood Banking and Transfusion Medicine: Basic Principle and Practice. 2nd ed. USA: Churchill Livingstone; 2007. p.735-6.
6. McLeod BC, Weinstein R, Winters JL, Szczepiorkowski ZM. Apheresis: Principles and Practice. 3rd ed. USA: AABB; 2010. p. 2-23.
7. Smith J, Burgstaler E. Blood component collection by apheresis. In: Roback JD, Combs MR, Grossman BJ, Hillyer ChD. Technical Manual. 17th ed. USA: AABB; 2008. p. 227-38.
8. Chegini A, Mirrezaie M, Poorreza M, Rezaie A, Anbardan A. Assessment of plateletphersis donation in Tehran Blood Center. Sci J Iran Blood Transfus Organ 2013; 10(2): 140-6. [Article in Farsi]
9. Sadeghi Neysiyani S , Amini-Kafiabad S. The effect of different types of plateletpheresis devices on the quality parameters of the produced platelet units. Sci J Iran Blood Transfus Organ 2024; 21(2): 169-84. [Article in Farsi]
10. Singh RP, Marwaha N, Malhotra P, Dash S. Quality assessment of platelet concentrates prepared by platelet rich plasma-platelet concentrate, buffy coat poor-platelet concentrate (BC-PC) and apheresis-PC methods. Asian J Transfus Sci 2009; 3(2): 86-94 [DOI:10.4103/0973-6247.53882] [PMID] []
11. Bertolini F, Rebulla P, Riccardi D, Cortellaro M, Ranzi ML, Sirchia G. Evaluation of platelet concentrates prepared from buffy coats and stored in a glucose‐free crystalloid medium. Transfusion 1989; 29(7): 605-9. [DOI:10.1046/j.1537-2995.1989.29789369678.x] [PMID]
12. Sadeghi Neysiyani S, Amini Kafi-Abad S. The effect of different types of plateletpheresis devices on the quality parameters of the produced platelet units. Sci J Iran Blood Transfus Organ 2024; 21(2): 169-84. [Article in Farsi]
13. Procházková R, Andrýs C, Hubáčková L, Krejsek J. Markers of platelet activation and apoptosis in platelet concentrates collected by apheresis. Transfus Apher Sci 2007; 37(2): 115-23. [DOI:10.1016/j.transci.2007.03.015] [PMID]
14. Macher S, Sipurzynski‐Budrass S, Rosskopf K, Rohde E, Griesbacher A, Groselj‐Strele A, et al. Function and activation state of platelets in vitro depend on apheresis modality. Vox Sang 2010; 99(4): 332-40. [DOI:10.1111/j.1423-0410.2010.01353.x] [PMID]
15. Xia Y, Li J, Bertino A, Kuter D. Thrombopoietin and the TPO receptorduring platelet storage. Transfusion 2000; 40(8): 976-87. [DOI:10.1046/j.1537-2995.2000.40080976.x] [PMID]
16. Ghasemi M, Turnbull T, Sebastian S, Kempson I. The MTT assay: utility, limitations, pitfalls, and interpretation in bulk and single-cell analysis. Int J Mol Sci 2021; 22(23): 12827. [DOI:10.3390/ijms222312827] [PMID] []
17. Hussein E. Clinical and quality evaluation of apheresis vs random‐donor platelet concentrates stored for 7 days. Transfus Med 2015; 25(1): 20-6. [DOI:10.1111/tme.12187] [PMID]
18. Tynngård N, Lindahl TL, Trinks M, Studer M, Berlin G. The quality of platelet concentrates produced by COBE Spectra and Trima Accel cell separators during storage for 7 days as assessed by in vitro methods. Transfusion 2008; 48(4): 715-22. [DOI:10.1111/j.1537-2995.2007.01610.x] [PMID]
19. Toora E, Kulkarni RG, Manivannan P, Sastry AS, Basavarajegowda A, Sahoo D. Quality assessment of platelet concentrates prepared by platelet-rich plasma, buffy-coat, and apheresis methods in a tertiary care hospital in South India: A cross-sectional study. Asian J Transfus Sci 2023; 17(2): 239-45. [DOI:10.4103/ajts.ajts_73_22] [PMID] []
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