Volume 22, Issue 2 (Summer 2025)
bloodj 2025, 22(2): 150-159 |
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Ashouri kafshgar E, Chegini A, Samiee S, Zadsar M. The Evaluation of Quality Parameters of Platelet Apheresis Products Obtained from the Fresenius COM.TEC Device during Storage. bloodj 2025; 22 (2) :150-159
URL: http://bloodjournal.ir/article-1-1563-en.html
URL: http://bloodjournal.ir/article-1-1563-en.html
Abstract: (198 Views)
A B S T R A C T
Background and Objectives
Platelets are small, nucleus-free cells vital for a variety of therapeutic interveutions. Ensuring the quality of transfused platelets is critical for optimal patient outcomes. Various devices are for separating platelet products from whole blood, each with unique characteristics. This study aimed to evaluate the Fresenius COM.TEC platelet apheresis machine by analyzing changes in pH, platelet count (PLT), Lactate dehydrogenase (LDH) levels, and platelet viability (MTT assay) across storage days 1, 3, and 5.
Materials and Methods
In this experimental study, 25 randomly selected platelet apheresis units from male donors were collected at the Tehran Blood Transfusion Center using the Fresenius COM.TEC device. The platelet products were stored at 20-24 °C in a shaker incubator. Samples were collected on storage days 1, 3, and 5 for evaluation of pH, LDH, PLT count, and platelet viability (MTT). All tests were conducted at the Blood Transfusion Research Center laboratory. Data analysis was performed using R software, and results with a p-values 0.05 were considered statistically significant.
Results
LDH levels were highest on day 5 and showed a significant increase throughout the storage period, with statistically significant differences between storage days. A consistent pH decline was also observed over time. MTT assay results indicated an transient increase on day 3, followed by a decline on day 5, with reaching statistical significance (p< 0.0001). in contrasts, platelet count did not exhibit any significant changes across all storage days (p> 0.05).
Conclusions
There was no significant change in Platelet counts in apheresis products collected via the Fresenius COM.TEC device during storage. However, the observed pH decrease and LDH increase on days 1, 3, and 5 are consistent with trends reproted in similar studies. Interpretation of MTT assay outcomes should be approaches with caution due to its limitations, underscoring the need for robust control.
Background and Objectives
Platelets are small, nucleus-free cells vital for a variety of therapeutic interveutions. Ensuring the quality of transfused platelets is critical for optimal patient outcomes. Various devices are for separating platelet products from whole blood, each with unique characteristics. This study aimed to evaluate the Fresenius COM.TEC platelet apheresis machine by analyzing changes in pH, platelet count (PLT), Lactate dehydrogenase (LDH) levels, and platelet viability (MTT assay) across storage days 1, 3, and 5.
Materials and Methods
In this experimental study, 25 randomly selected platelet apheresis units from male donors were collected at the Tehran Blood Transfusion Center using the Fresenius COM.TEC device. The platelet products were stored at 20-24 °C in a shaker incubator. Samples were collected on storage days 1, 3, and 5 for evaluation of pH, LDH, PLT count, and platelet viability (MTT). All tests were conducted at the Blood Transfusion Research Center laboratory. Data analysis was performed using R software, and results with a p-values 0.05 were considered statistically significant.
Results
LDH levels were highest on day 5 and showed a significant increase throughout the storage period, with statistically significant differences between storage days. A consistent pH decline was also observed over time. MTT assay results indicated an transient increase on day 3, followed by a decline on day 5, with reaching statistical significance (p< 0.0001). in contrasts, platelet count did not exhibit any significant changes across all storage days (p> 0.05).
Conclusions
There was no significant change in Platelet counts in apheresis products collected via the Fresenius COM.TEC device during storage. However, the observed pH decrease and LDH increase on days 1, 3, and 5 are consistent with trends reproted in similar studies. Interpretation of MTT assay outcomes should be approaches with caution due to its limitations, underscoring the need for robust control.
Type of Study: Research |
Subject:
Biotechnology
References
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