Journal of Iranian Blood Transfusion

  Abstract

 Background and Objectives

  Although the French-American-British (FAB) Classification system is the basis for the diagnosis and treatment of AML, it has its own limitations. During recent years immunophenotyping by flow cytometry is widely used for the identification of AML'S subtypes. Immunophenotyping has been especially helpful in discrimination of AML with monocytic differentiation (M4-M5) from nonmonocytic subtypes (M0- M1- M2- M3- M6- M7). However several studies have indicated that CD14 mostly is negative when applied to leukemias with monocyte differentiation.

  Materials and Methods 

  Some studies have shown that CD64 (FC g RI) is an early and specific myelo-monoid marker. In this study we evaluated CD14 and CD64 antibodies to identify cells of monocytic lineage in 216 cases of AML who were referred to IBTO flow cytometry laboratory. These monoclonal antibodies prepared by DAKO company and were conjugated with Phyco Erythrin. The samples were analysed by Epics-x1 Flow cytometer. The markers were considered positive if 20% or more of the cells expressed it. The Chi-square test was also used in SPSS software.

 Results

  Results revealed that CD64 was highty specific (88%) and sensitive (67%) and CD14 was highty specific (96%) but not sensitive (31%) with >95% confidence rate. These results are compatible with other studies.

 Conclusions

  Finally because of high sensitivity of CD64, it should be considered in all of immunophenotyping protocols as a sensitive and specific marker for monoid cells.