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:: Volume 4, Issue 3 (Autumn 2007) ::
Sci J Iran Blood Transfus Organ 2007, 4(3): 223-229 Back to browse issues page
Evaluation of metabolic parameters in pooled platelet stored for 5 days
M. Mahmoodian Shooshtari, A.A. Dawatgar, M. Aghaipour, Z. Sharifi
Abstract:   (17636 Views)



 Background and Objectives

 Platelets prepared by random donor method were studied to measure metabolic parameters during 5 days of storage.


 Materials and Methods

 The random donor platelets were obtained by two-phase centrifugation during the first phase the derived component was platelet-rich plasma from which platelet concentrate was obtained. They were then stored at 22 ° C on an agitator. Four pooled platelets (each pool containing 5 platelet concentrates from random blood donors) were examined. Platelet count, LDH (Lactate Dehydrogenize), changes in pH value, PO2, PCO2, O2 sat, O2:CO2, glucose and lactate concentrations were compared on the first and fifth day.



 The average of pooled- platelet count in fresh units (day 1) was 2.7 × 1011 and in stored units (day 5) 2.1 × 1011. In stored units, platelet counts were lower as compared with fresh units. This indicates of platelet elimination during platelet storage. The pH values for all random pooled platelets were almost stable and no significant changes were observed. Changes between pH values of samples from the first day of collection and those of the fifth day of collection were very small. In fact, all pH values fell within the range of 7.4. Reduction in PO2 level in comparison to PCO2 level was sharply lower. In average, PO2 reduction in random pooled platelets on the fifth day was 12.4 ml Hg and it was 16.8 ml Hg for PCO2. The average O2/CO2 ratio on the first day was 2.7 and this ratio on the fifth day was 3.6 which shows an increase of 1.33% in the O2/CO2 ratio on the fifth day. In fact, PO2 level was higher than PCO2 thus contributing to pH stability. The concentration of Lactate dehydrogenizers (LDH) and Lactate considerably increased on the fifth day. The level of glucose decreased gradually from 24.8 to 21.5 m mol/l.



 The results indicate that the selection of suitable bags for platelets collection and storage can keep O2/CO2 ratio significantly higher even after the fifth day of collection it can contribute to the preservation and storage of platelets with acceptable quality.


 Key words : Platelet, pH, LDH


Keywords: Platelet, pH, LDH
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Type of Study: Research | Subject: Hematology
Published: 2014/08/17
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Mahmoodian Shooshtari M, Dawatgar A, Aghaipour M, Sharifi Z. Evaluation of metabolic parameters in pooled platelet stored for 5 days. Sci J Iran Blood Transfus Organ 2007; 4 (3) :223-229
URL: http://bloodjournal.ir/article-1-177-en.html

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Volume 4, Issue 3 (Autumn 2007) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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