Abstract

 Background and Objectives

 Platelets prepared by random donor method were studied to measure metabolic parameters during 5 days of storage.

 Materials and Methods

 The random donor platelets were obtained by two-phase centrifugation during the first phase the derived component was platelet-rich plasma from which platelet concentrate was obtained. They were then stored at 22 ° C on an agitator. Four pooled platelets (each pool containing 5 platelet concentrates from random blood donors) were examined. Platelet count, LDH (Lactate Dehydrogenize), changes in pH value, PO₂, PCO₂, O₂ sat, O₂:CO₂, glucose and lactate concentrations were compared on the first and fifth day.

 Results

 The average of pooled- platelet count in fresh units (day 1) was 2.7 × 10¹¹ and in stored units (day 5) 2.1 × 10¹¹. In stored units, platelet counts were lower as compared with fresh units. This indicates of platelet elimination during platelet storage. The pH values for all random pooled platelets were almost stable and no significant changes were observed. Changes between pH values of samples from the first day of collection and those of the fifth day of collection were very small. In fact, all pH values fell within the range of 7.4. Reduction in PO₂ level in comparison to PCO₂ level was sharply lower. In average, PO₂ reduction in random pooled platelets on the fifth day was 12.4 ml Hg and it was 16.8 ml Hg for PCO₂. The average O₂/CO₂ ratio on the first day was 2.7 and this ratio on the fifth day was 3.6 which shows an increase of 1.33% in the O₂/CO₂ ratio on the fifth day. In fact, PO₂ level was higher than PCO₂ thus contributing to pH stability. The concentration of Lactate dehydrogenizers (LDH) and Lactate considerably increased on the fifth day. The level of glucose decreased gradually from 24.8 to 21.5 m mol/l.

 Conclusions

 The results indicate that the selection of suitable bags for platelets collection and storage can keep O₂/CO₂ ratio significantly higher even after the fifth day of collection it can contribute to the preservation and storage of platelets with acceptable quality.

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 Key words : Platelet, pH, LDH