Ethics code: IR.TMI.RCE.1398.014
Fateme Roshanzamir * 
, Sedigheh Amini-kafiabad , Javad Mohajer Ansari , Mahin Nikougoftar Zarif , Ali Arabkhazaeli , Mahshid Mohammadipour
, Sedigheh Amini-kafiabad , Javad Mohajer Ansari , Mahin Nikougoftar Zarif , Ali Arabkhazaeli , Mahshid Mohammadipour
Abstract: (15 Views)
A B S T R A C T
Background and Objectives
Approximately 85-90 million red blood cell (RBC) components are transfused worldwide each year. Among these, leukoreduced RBC units particularly important for patients chronically need blood transfusion. Leukocyte filtration is commonly performed using two methods: whole-blood filtration (WBF) and red blood cell filtration (RCF). Given the potential impact of processing techniques on product quality, this study was designed to compare the effects of WBF and RCF on product quality indicators. In this study, an attempt has been made to eliminate background variables by equalizing conditions in order to focus on the effect of the filtration method.
Materials and Methods
Twelve whole blood units were divided into two equal parts, with each part filtered using WBF or RCF filters. Samples were taken on days 2, 14, 28, and 42 and they were measured for microvesicle (MVs) concentration, erythrocyte indices, free hemoglobin (Hb) levels, osmotic fragility (OFT), pH, and lactate dehydrogenase (LDH) levels. Data were analyzed using SPSS software, with two-group independent t-tests and ANOVA with repeated measures.
Results
Over storage time, both product groups showed a significant increase in microvesicle concentration, LDH, OFT, free hemoglobin, MCV, and hematocrit, alongside a decrease in pH. The differences between groups were statistically significant at some sampling intervals for microvesicle concentration, pH, LDH, free hemoglobin, and OFT (p< 0.05).
Conclusions
Given the homogenization of conditions and the relative elimination of individual variables, it seems that the components prepared with the WBF method differ in quality from those prepared with the RCF method. However, despite the observed differences, the highest level of significance for the indicators was on the last day of storage period. These findings indicate that, both product groups remained within quality limits for transfusion.
Background and Objectives
Approximately 85-90 million red blood cell (RBC) components are transfused worldwide each year. Among these, leukoreduced RBC units particularly important for patients chronically need blood transfusion. Leukocyte filtration is commonly performed using two methods: whole-blood filtration (WBF) and red blood cell filtration (RCF). Given the potential impact of processing techniques on product quality, this study was designed to compare the effects of WBF and RCF on product quality indicators. In this study, an attempt has been made to eliminate background variables by equalizing conditions in order to focus on the effect of the filtration method.
Materials and Methods
Twelve whole blood units were divided into two equal parts, with each part filtered using WBF or RCF filters. Samples were taken on days 2, 14, 28, and 42 and they were measured for microvesicle (MVs) concentration, erythrocyte indices, free hemoglobin (Hb) levels, osmotic fragility (OFT), pH, and lactate dehydrogenase (LDH) levels. Data were analyzed using SPSS software, with two-group independent t-tests and ANOVA with repeated measures.
Results
Over storage time, both product groups showed a significant increase in microvesicle concentration, LDH, OFT, free hemoglobin, MCV, and hematocrit, alongside a decrease in pH. The differences between groups were statistically significant at some sampling intervals for microvesicle concentration, pH, LDH, free hemoglobin, and OFT (p< 0.05).
Conclusions
Given the homogenization of conditions and the relative elimination of individual variables, it seems that the components prepared with the WBF method differ in quality from those prepared with the RCF method. However, despite the observed differences, the highest level of significance for the indicators was on the last day of storage period. These findings indicate that, both product groups remained within quality limits for transfusion.
Keywords: Erythrocyte Transfusion, Blood Component Removal, Storage Lesions, Red Blood Cells, Cell-derived Microparticles, Cell Separation
Type of Study: Research |
Subject:
Blood Transfusion
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