Volume 22, Issue 2 (Summer 2025)
bloodj 2025, 22(2): 123-136 |
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Rahimi Sabet Z, Ghobeh M, Khorshidfar M, Deyhim M R. Comparative Analysis of Oxidative Damage and Metabolic Parameters in Red Blood Cells Stored in CPDA1 and CPD+SAGM. bloodj 2025; 22 (2) :123-136
URL: http://bloodjournal.ir/article-1-1578-en.html
URL: http://bloodjournal.ir/article-1-1578-en.html
Abstract: (256 Views)
A B S T R A C T
Background and Objectives
During storage, Red Blood Cells (RBCs) undergo a series of morphological and functional changes that diminish their survival and function, and collectively are known as Red Blood Cell storage lesion. Oxidative damage and metabolic changes in RBCs are the main causes of this lesion. This study aimed to evaluate RBC storage lesion using oxidative and metabolic parameters in two distinct preservation systems: CPDA1 and CPD combined with SAGM.
Materials and Methods
In this experimental study, carried out at the Blood Transfusion Research Center, five RBC units containing CPDA1 and five with CPD+SAGM were selected through simple random sampling. Oxidative and metabolic parameters were evaluated over a 42-day storage period, with evaluations conducted on days 0, 2, 7, 14, 21, 28, 35, and 42. For each sampling point, parameter measurements were performed in duplicate. Independent t-tests and Mann-Whitney tests were used to compare variables at each time between groups. A two-way variance test with repeated measures was employed point to evaluate the trend of changes within the group over time.
Results
The findings indicated that the activity of lactate dehydrogenase (LDH) and the concentration of lactate exhibited a markedly lower increase in the CPD+SAGM group compared to the CPDA1 group (p<0.05). Furthermore, the glucose concentration and pH exhibited a less reduction in the CPD+SAGM group than in the CPDA1 group (p<0.05). In a pairwise group, comparison of malondialdehyde (MDA) and total oxidant concentration showed a less increase in the CPD+SAGM group when compared to the CPDA1 group across various days of RBC storage, particularly from the day 7 onward (p<0.05).
Conclusions
The results of this study showed that SAGM more effectively mitigates the RBC storage lesion compared to CPDA1 by maintaining metabolic activity and reducing oxidative damage. This may contribute to better preservation of RBC survival and improvement of the quality during storage. However, further studies are required at both in vitro and in vivo levels.
Background and Objectives
During storage, Red Blood Cells (RBCs) undergo a series of morphological and functional changes that diminish their survival and function, and collectively are known as Red Blood Cell storage lesion. Oxidative damage and metabolic changes in RBCs are the main causes of this lesion. This study aimed to evaluate RBC storage lesion using oxidative and metabolic parameters in two distinct preservation systems: CPDA1 and CPD combined with SAGM.
Materials and Methods
In this experimental study, carried out at the Blood Transfusion Research Center, five RBC units containing CPDA1 and five with CPD+SAGM were selected through simple random sampling. Oxidative and metabolic parameters were evaluated over a 42-day storage period, with evaluations conducted on days 0, 2, 7, 14, 21, 28, 35, and 42. For each sampling point, parameter measurements were performed in duplicate. Independent t-tests and Mann-Whitney tests were used to compare variables at each time between groups. A two-way variance test with repeated measures was employed point to evaluate the trend of changes within the group over time.
Results
The findings indicated that the activity of lactate dehydrogenase (LDH) and the concentration of lactate exhibited a markedly lower increase in the CPD+SAGM group compared to the CPDA1 group (p<0.05). Furthermore, the glucose concentration and pH exhibited a less reduction in the CPD+SAGM group than in the CPDA1 group (p<0.05). In a pairwise group, comparison of malondialdehyde (MDA) and total oxidant concentration showed a less increase in the CPD+SAGM group when compared to the CPDA1 group across various days of RBC storage, particularly from the day 7 onward (p<0.05).
Conclusions
The results of this study showed that SAGM more effectively mitigates the RBC storage lesion compared to CPDA1 by maintaining metabolic activity and reducing oxidative damage. This may contribute to better preservation of RBC survival and improvement of the quality during storage. However, further studies are required at both in vitro and in vivo levels.
Type of Study: Research |
Subject:
Biochemistry
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