[Home ] [Archive]   [ فارسی ]  
:: Main :: About us :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 3, Issue 4 (Winter 2007) ::
Sci J Iran Blood Transfus Organ 2007, 3(4): 325-331 Back to browse issues page
Determination of Alpha/Beta ratio in healthy individuals (18-45 years old) with normal hematological indices (MCV≥ 80, MCH≥ 27) and normal HbA2 by cation exchange chromatography
Ladan Hosseini Gohari , Isa Noormohammadi, Ali Akbar Sharafi Tafresi Moghadam, Leila Mostaan, Aneti Drousiotou
Abstract:   (23762 Views)

 

  Abstract

 Background and Objectives

 For a successful prevention program, globin chain synthesis, as a complementary test beside DNA analysis, is necessary. So, it is important that each laboratory establishes its own reference range for the classification of thalassemia syndromes by globin chain synthesis. Globin chain synthesis is a relatively complex test introduced in the study of thalassemia syndromes as a reference method. The technique is also useful for variant chain identification.This study aims to stablish the method of globin chain synthesis to determine a / b chain ratio in healthy individuals.

 

 Materials and Methods

 In this study globin chain analysis was performed on 30 healthy laboratory personnels with normal HbA2 and normal hematological indices. In this method a reticulocyte-rich sample is incubated with a mixture of amino acids, one of which (leucine) radioactively labelled. After washing the excess radioactivity and precipitating the globin, different chains are separated by cation exchange chromatography.

 

 Results

 The mean a / b ratio was 1.045 ± 0.12 (mean ± 1SD) in healthy subjects. Our findings were in agreement with those of the other investigators in the world.

 

 Conclusions

 In any screening program, diagnostic problems will arise that can not be solved without biosynthetic studies. The Clegg and Weatherall method has been proved to be very reliable and reproducible, but time-consuming (requiring four days). New methods like reverse phase HPLC are now available for chain separation. Therefore, according to the procedures each laboratory should determine its own reference range.

  

 Key words : a globin, b globin, Hemoglobin, Normal range, Ion exchange chromatography

 

Keywords: Alpha globin, Beta globin, Hemoglobin, Normal range, Ion exchange chromatography
Full-Text [PDF 319 kb]   (6503 Downloads)    
Type of Study: Research | Subject: General
Published: 2014/08/18
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hosseini Gohari L, Noormohammadi I, Sharafi Tafresi Moghadam A A, Mostaan L, Drousiotou A. Determination of Alpha/Beta ratio in healthy individuals (18-45 years old) with normal hematological indices (MCV≥ 80, MCH≥ 27) and normal HbA2 by cation exchange chromatography. Sci J Iran Blood Transfus Organ. 2007; 3 (4) :325-331
URL: http://bloodjournal.ir/article-1-121-en.html


Volume 3, Issue 4 (Winter 2007) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
Persian site map - English site map - Created in 0.04 seconds with 31 queries by YEKTAWEB 4312