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Sci J Iran Blood Transfus Organ 2016, 13(2): 130-137 Back to browse issues page
Study of quantitative and qualitative gene expression of lipoprotein lipase before and after differentiation of human bone marrow mesenchymal stem cells into adipocytes
S.R. Razavi Babaheydari, K. Mousavi Hosseini Dr. , A. Atashi Dr., Sh. Esmaeeili
Keywords: Key words: Mesenchymal Stromal Cells, Adipocytes, Lipoprotein Lipase, Cell Differentiation
Full-Text [PDF 468 kb]   (792 Downloads)     |   Abstract (HTML)  (4365 Views)
Type of Study: Research | Subject: Stem cells
Published: 2016/06/15
Full-Text:   (855 Views)
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Original  Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2016; 13(2): 130-137
 
 

Study of quantitative and qualitative gene expression of lipoprotein lipase before and after differentiation of human bone marrow mesenchymal stem cells into adipocytes
Razavi Babaheidary S.R.1, Mousavi Hosseini K.1, Atashi A.2, Esmaeeili Sh.2
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
 
 
Abstract
Background and Objectives
Serum TG plays an important role in carcinogenesis and the LPL as a key enzyme that hydrolyses plasma TG involves in this process. Abnormal regulation and/or expression of LPL play roles in many human diseases such as atherosclerosis. Serum TG levels increase the risk of chylomicronaemia, obesity and type 2 diabetes; thus, in this study we reported LPL expression before and after BM-MSCs differentiation into adipocyte under controlled conditions.
                                                                                                                                                                                                   
Materials and Methods
MSCs from human bone marrow were isolated from 3 healthy people and then differentiated into adipocytes by chemical materials for 14 days. Then, total RNA was extracted and cDNA was synthesized and finally RT-PCR and Real Time PCR analysis were performed for LPL gene.
 
Results
After 14 days, the analysis of morphology with invert microscopy indicated that BM-MSCs have the capacity of differentiation into adipocytes. BM-MSCs serving as the negative control do not have any expression of LPL while in BM-MSCs derived-adipocytes there has been the significant expression of LPL.
 
Conclusions
BM-MSCs can differentiate into either adipocytes or osteoblastic cells; LPL has the important role in the differentiation decision for either one. The inhibition or stimulation of LPL expression can be used for the treatment of various diseases including prostate cancer, aplastic anemia, and osteoporosis.
 
Key words: Mesenchymal Stromal Cells, Adipocytes, Lipoprotein Lipase, Cell Differentiation
 
 
 
Received:  24 Sep 2015
Accepted: 30 Jan 2016
 
 

Correspondence: Mousavi Hosseini K., PhD in Medicinal Chemistry. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052160; Fax: (+9821) 88601599
E-mail:
mkmousavi@yahoo.com
Correspondence: Atashi A., PhD of Hematology & Blood Banking. Assistant Professor of Faculty of Medical Sciences, Tarbiat Modares University.
Postal Code: 14115-111, Tehran, Iran. Tel: (+9821) 82883579; Fax: (+9821) 82884507
E-mail:
atashia@modares.ac.ir
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Razavi Babaheydari S, Mousavi Hosseini K, Atashi A, Esmaeeili S. Study of quantitative and qualitative gene expression of lipoprotein lipase before and after differentiation of human bone marrow mesenchymal stem cells into adipocytes. Sci J Iran Blood Transfus Organ. 2016; 13 (2) :130-137
URL: http://bloodjournal.ir/article-1-993-en.html


Volume 13, Issue 2 (Summer 2016) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
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