Volume 12, Issue 2 (Summer 2015)                   Sci J Iran Blood Transfus Organ 2015, 12(2): 153-162 | Back to browse issues page

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Mehrpoori M, Hosseini E, Amini Kafi-Abad S. The effect of pre-storage leukoreduction on the levels of expression and shedding of the pro-inflammatory molecule P-Sel in random PRP platelets. Sci J Iran Blood Transfus Organ 2015; 12 (2) :153-162
URL: http://bloodjournal.ir/article-1-942-en.html
The effect of pre-storage leukoreduction on the levels of expression and shedding of the pro-inflammatory molecule P-Sel in random PRP platelets
M. Mehrpoori , E. Hosseini , S. Amini Kafi-Abad
Keywords: Key words: P-Selectin, Leukocyte Reduction Procedures, Platelet Activation
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Original  Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2015; 12(2): 153-162
 
 

The effect of pre-storage leukoreduction on the levels
of expression and shedding of the pro-inflammatory
molecule P-Sel in random PRP platelets
 
Mehrpoori M.1, Hosseini E.1, Amini Kafi-Abad S.1, Ghasemzadeh M.1
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
Abstract
Background and Objectives
During storage, functional activity of platelets is affected by platelet storage lesion (PSL). The over-expression of P-Selectin is one of the most important evidence of platelet activation, thereby monitoring P-Sel expression and shedding can be considered as an index for performance in platelet concentrates.
 
Materials and Methods
Ten PRP-platelet concentrates obtained from Tehran Regional Educational Blood Transfusion Center were subdivided into two groups of usual platelets and leuko-reduced platelets following pre-storage filtration. Flow-cytometery and ELISA were used to evaluate P-Sel expression and shedding, respectively. Data were analyzed by one way-Anova and Mann-Whitney U tests using Graphpad software.
 
Results
In filtered products, P-Sel expression on days 1, 3 and 5 after storage were 13.5 ± 1.8%, 15.2 ± 1.2% and 27.4 ± 2.5% , respectively. The results showed a sinificant increase of expression in day 5, compared to that in day 1 and 3. In non-filtered products, P-Sel expression on days 1, 3 and 5 were 7.4 ± 1.7%, 21 ± 2%, and 10 ± 2.2%, respectively. Increased expression in day 3 was followed by a significant decrease in day 5, that might be due to the loss of receptors via shedding and/or microparticulation during platelet post-activation. The levels of soluble P-Sel in filtered products were significantly less than unfiltered products in day 3.
 
Conclusions
Considering the expression and shedding of P-Sel, this study proposes that the leuko-reduction process favor less PSL effect during the storage of platelet products.
 
Key words: P-Selectin, Leukocyte Reduction Procedures, Platelet Activation
 
 
 
 
 
 
 
 
Received: 16  Feb 2015
Accepted:  6  Jun 2015
 
 
 

Correspondence: Ghasemzadeh M., PhD of Biochemistry. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box:14665-1157, Tehran, Iran. Tel: (+9821) 88601501-20 ; Fax: (+9821) 88601599
E-mail:
mehran1476@yahoo.com
Type of Study: Research | Subject: Blood Transfusion
Published: 2015/07/5
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