Volume 12, Issue 2 (Summer 2015)                   Sci J Iran Blood Transfus Organ 2015, 12(2): 111-124 | Back to browse issues page

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Nasiri F, Amiri F, Mohammadipour M, Molaei S, Habibi Roudkenar M, Jalili M. H2O2-preconditioned mesenchymal stem cell regenerative effects on acute liver failure mice. Sci J Iran Blood Transfus Organ 2015; 12 (2) :111-124
URL: http://bloodjournal.ir/article-1-916-en.html
H2O2-preconditioned mesenchymal stem cell regenerative effects on acute liver failure mice
F. Nasiri , F. Amiri , M. Mohammadipour , S. Molaei , M. Habibi Roudkenar , M.A. Jalili *
Keywords: Key words : Ischemic Preconditioning, Oxidative Stress, CCL4, Acute Liver Failure
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Original  Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2015; 12(2): 111-124
 
 

H2O2-preconditioned mesenchymal stem cell regenerative
effects on acute liver failure mice
 
Nasiri F.1, Amiri F.1, Mohammadipour M.1, Molaei S.1, Habibi Roudkenar M.1, Jalili M.A.1
 
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
 
 
 
Abstract
Background and Objectives
Mesenchymal stem cells (MSCs) are attractive cells for cell therapy. However, the efficacy of MSCs is limited because of low survival rate following translation. In this study, MSCs were preconditioned with sub-lethal doses of H2O2. The therapeutic potential of preconditioning MSCs was examined on acute liver failure in mice.
 
Materials and Methods
In the present experimental study, MSCs were isolated from bone marrow aspirate. Cells from passage four were treated with  different concentrations of H2O2 for 24 hrs, and then were recovered in fresh medium for 7 hrs followed by exposure to lethal doses of H2O2. Cell viability was measured with WST-1 assay. In the in vivo phase, acute liver failure was  induced by CCl4; then, the regenerative potential of preconditioned-MSCs was evaluated using biochemical as well as histological methods.
 
Results
Survival rates were higher in the engrafted mice with preconditioned-MSCs in comparison to those who received normal MSCs. Three days after cell therapy liver enzymes reached the normal level in engrafted group with preconditioned-MSCs. Interestingly, histological results revealed a significant improvement in liver regeneration potential in transplanted groups with preconditioned-MSCs.
 
Conclusions
Preconditioning of MSCs with H2O2 not only enhances their survival but also increases the efficacy of MSC-based cell therapy in liver acute failure.
 
Key words: Ischemic Preconditioning, Oxidative Stress, CCl4, Acute Liver Failure
 
 
 
 
 
 
Received:  2 Nov 2014
Accepted: 3 Mar 2015
 
 
 

Correspondence: Jalili MA., PhD of Medicinal Chemistry. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052155; Fax: (+9821) 88601599
E-mail: m.jalili@ibto.ir
Type of Study: Research | Subject: Stem cells
Published: 2015/07/5
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