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Mashayekhi A, Shahbazi S. Evaluation of the association between FGBrs1800790 ‎‏ ‏and plasma fibrinogen levels. Sci J Iran Blood Transfus Organ 2015; 11 (1) :48-55
URL: http://bloodjournal.ir/article-1-855-en.html
Evaluation of the association between FGBrs1800790 ‎‏ ‏and plasma fibrinogen levels
A. Mashayekhi , Sh. Shahbazi *
Keywords: Key words: Fibrinogen, Genetic Polymorphism, RFLP
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Sci J Iran Blood Transfus Organ 2014; 11(1): 48-55
 
Original Article
 
 

Evaluation of the association between FGBrs1800790 ‎‏ ‏
and plasma fibrinogen levels
 
 
Mashayekhi A.1, Shahbazi Sh.1
 
 
1Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
 
 
 
Abstract
Background and Objectives
Fibrinogen, coagulation factor 1, is a soluble plasma glycoprotein which converts thrombin to fibrin fibers in the coagulation cascade. Various polymorphisms located on beta chain gene, FGB, have been studied. The promoter – G455A (rs1800790) polymorphism had been shown to be significantly associated with fibrinogen plasma levels. In this study, the frequency of rs1800790‎ polymorphism has been assessed and the correlation was evaluated between this variant and fibrinogen plasma levels.
 
Materials and Methods
Whole blood samples were collected for DNA isolation. To perform ‎rs1800790‎ genotyping, the region encompassing the HaeIII restriction site was amplified using specific primers. The plasma levels of fibrinogen were measured and the obtained data were analyzed by SPSS software.
 
Results
The levels of plasma fibrinogen has shown a significant correlation with FGBrs1800790‎ genotype (p-value = 0.021‎‏; ‏CI = 0‎.347‎‏-‎‎3.253). Minor allele frequency was estimated to be 0.28 which was consistent with the predicted value.
 
Conclusions 
The significant correlation between fibrinogen and FGBrs1800790‎ genotype emphasizes the importance of this variant in the modulation of fibrinogen plasma concentrations. Since FGBrs1800790‎ contributes to the gene expression and environmental risk factors, it is important to be noticed in the cardiovascular disease.
 
Key words: Fibrinogen, Genetic Polymorphism, RFLP
 
 
 
 
 
 
 
Received: 13 May 2013
Accepted:  8 Oct  2013
 
 
 

Correspondence: Shahbazi Sh., PhD of Genetics. Assistant Professor of Faculty of Medical Sciences, Tarbiat Modares University.
P.O.Box: 14115-331, Tehran, Iran. Tel: (+9821) 82884556; Fax: (+9821) 82884555
E-mail:
sh.shahbazi@modares.ac.ir
Type of Study: Research | Subject: Blood transfusion medicine
Published: 2014/03/16
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