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Sci J Iran Blood Transfus Organ 2015; 11(4): 318-324
|
Evaluation of natural autoantibody against red
blood cells in healthy individuals
Goudarzi S.1,2, Pourfathollah A.A.1,2, Moghaddam M.2, Zeidi M.2,3, Masaee M.1
1Faculty of Medical Sciences,Tarbiat Modares University, Tehran, Iran
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3Tehran Regional Educational Blood Transfusion Center, Tehran, Iran
Abstract
Background and Objectives
Natural autoantibodies (N-Auto-Abs) are a class of antibodies that are formed without any previous provocation against their own. Currently an important role has been proposed for these antibodies in immune homeostasis; as we can study them as biological markers to predict the onset of the disease. For the first time, we studied the prevalence of N-Auto-Abs against RBC antigens as well as the prevalence of cold antibodies in blood donors.
Materials and Methods
Three hundred healthy individuals who donated at Tehran Blood Transfusion Center were selected randomly; their blood samples were collected including 289 (96.3%) males and 11 (3.7%) female. Their age range is within 17-64 years. EDTA plasma was evaluated for the study of N-Auto-Abs that reacted at 4C.
Results
28 individuals (9.34%) showed positive reaction with their own cell suspension. In addition, 55 individuals (18.34%) were positive for cold antibodies.
Conclusions
Twenty eight individuals (9.34%) showed positive reaction with their own cell suspension. In addition, 55 individuals (18.34%) were positive for cold antibodies.
Key words: Red Blood Cells, Blood Donors, Autoantibodies
Received: 13 Jul 2013
Accepted: 5 Nov 2013
Correspondence: Pourfathollah AA., PhD of Immunology. Professor of Faculty of Medical Sciences of Tarbiat Modares University and Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box:14115-111, Tehran, Iran. Tel: (+9821) 8801101; Fax: (+9821) 88013030
E-mail:
pourfa@modares.ac.ir