Volume 21, Issue 3 (Autumn 2024)                   Sci J Iran Blood Transfus Organ 2024, 21(3): 197-206 | Back to browse issues page

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Mohammadali F, Abroun S, Atashi A. The effect of mild hypoxia on Stemness of CD34+ cells isolated from umbilical cord blood cocultured with human bone marrow mesenchymal stem cells. Sci J Iran Blood Transfus Organ 2024; 21 (3) :197-206
URL: http://bloodjournal.ir/article-1-1535-en.html
The effect of mild hypoxia on Stemness of CD34+ cells isolated from umbilical cord blood cocultured with human bone marrow mesenchymal stem cells
F. Mohammadali , S. Abroun * , A. Atashi
Keywords: Cord Blood, Hematopoietic Stem Cells, Mesenchymal Stem Cell, Coculture, Hypoxia
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Sci J Iran Blood Transfus Organ 2024;21 (3): 197-206
Original Article
 
The effect of mild hypoxia on Stemness of CD34+ cells isolated from umbilical cord blood cocultured with human bone marrow mesenchymal stem cells

Mohammadali F.1, Abroun S.2, Atashi A.3


1Blood Transfusion Research Center, High Institute For Research and Education in Transfusion Medicine, Tehran, Iran
2Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran


Abstract
Background and Objectives
Cord blood (CB) is a rich source of  Hematopoietic stem cells (HSCs). Beside the advantage the main disadvantages of CB are  the limited number of stem cells and delayed engraftment. Identifying strategies to enhance expansion and maintain stemness of HSCs can improve transplant efficiency. The goal of this study was to examine different culture conditions on HOXB4, c-Myc, Nanog, Sox2  gene expression of CB-HSCs.

Materials and Methods
In this interventional study, human cord blood CD34+ HSC, were cultured in the serum-free me­dium supplemented with cytokines (TPO, FLT3L, SCF) with/without  bone marrow mesenchymal stem cell (MSC) in 21% O2  and 5% O2 for 7 days. In day 7 the expression of, HOXB4, c-Myc, Nanog, Sox2  genes were evaluated by Real time PCR. The data analyzed using the ANOVA test  and Value < 0.05 were considered statistically significant.

Results
Highest rates of  HOXB4, c-Myc, Nanog, SOX2 mRNA were seen in coculture of HSC with bone marrow  MSC at 5% O2. Our findings demonstrated  statistically significant increase of expansion and stemness markers in 5% O2 tension versus 21%.

Conclusions 
Bone Marrow (BM) -MSC and 5% O2 combination enhanced stemness of HSC  and better mimicked the niche microenvironment conditions.

Key words: Cord Blood, Hematopoietic Stem Cells, Mesenchymal Stem Cell, Coculture, Hypoxia





Received:  8   Jun 2024
Accepted: 27 Aug 2024



Correspondence: Abroun S., PhD of Hematology and Blood Banking. Professor of Tarbiat Modares University.
P.O.Box: 14155-331, Tehran, Iran. Tel: (+9821) 82883860; Fax: (+9821) 82884507
E-mail: Abroun@modares.ac.ir
Type of Study: Research | Subject: Stem cells
Published: 2024/10/1
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