Volume 21, Issue 3 (Autumn 2024)
Sci J Iran Blood Transfus Organ 2024, 21(3): 197-206 |
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Mohammadali F, Abroun S, Atashi A. The effect of mild hypoxia on Stemness of CD34+ cells isolated from umbilical cord blood cocultured with human bone marrow mesenchymal stem cells. Sci J Iran Blood Transfus Organ 2024; 21 (3) :197-206
URL: http://bloodjournal.ir/article-1-1535-en.html
URL: http://bloodjournal.ir/article-1-1535-en.html
Abstract: (432 Views)
Abstract
Background and Objectives
Cord blood (CB) is a rich source of Hematopoietic stem cells (HSCs). Beside the advantage the main disadvantages of CB are the limited number of stem cells and delayed engraftment. Identifying strategies to enhance expansion and maintain stemness of HSCs can improve transplant efficiency. The goal of this study was to examine different culture conditions on HOXB4, c-Myc, Nanog, Sox2 gene expression of CB-HSCs.
Materials and Methods
In this interventional study, human cord blood CD34+ HSC, were cultured in the serum-free medium supplemented with cytokines (TPO, FLT3L, SCF) with/without bone marrow mesenchymal stem cell (MSC) in 21% O2 and 5% O2 for 7 days. In day 7 the expression of, HOXB4, c-Myc, Nanog, Sox2 genes were evaluated by Real time PCR. The data analyzed using the ANOVA test and Value < 0.05 were considered statistically significant.
Results
Highest rates of HOXB4, c-Myc, Nanog, SOX2 mRNA were seen in coculture of HSC with bone marrow MSC at 5% O2. Our findings demonstrated statistically significant increase of expansion and stemness markers in 5% O2 tension versus 21%.
Conclusions
Bone Marrow (BM) -MSC and 5% O2 combination enhanced stemness of HSC and better mimicked the niche microenvironment conditions.
Background and Objectives
Cord blood (CB) is a rich source of Hematopoietic stem cells (HSCs). Beside the advantage the main disadvantages of CB are the limited number of stem cells and delayed engraftment. Identifying strategies to enhance expansion and maintain stemness of HSCs can improve transplant efficiency. The goal of this study was to examine different culture conditions on HOXB4, c-Myc, Nanog, Sox2 gene expression of CB-HSCs.
Materials and Methods
In this interventional study, human cord blood CD34+ HSC, were cultured in the serum-free medium supplemented with cytokines (TPO, FLT3L, SCF) with/without bone marrow mesenchymal stem cell (MSC) in 21% O2 and 5% O2 for 7 days. In day 7 the expression of, HOXB4, c-Myc, Nanog, Sox2 genes were evaluated by Real time PCR. The data analyzed using the ANOVA test and Value < 0.05 were considered statistically significant.
Results
Highest rates of HOXB4, c-Myc, Nanog, SOX2 mRNA were seen in coculture of HSC with bone marrow MSC at 5% O2. Our findings demonstrated statistically significant increase of expansion and stemness markers in 5% O2 tension versus 21%.
Conclusions
Bone Marrow (BM) -MSC and 5% O2 combination enhanced stemness of HSC and better mimicked the niche microenvironment conditions.
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