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Investigating the apoptotic and anti-proliferative effects of Juniperus excelsa fractions on Acute Lymphoblastic leukemia cell line (Nalm-6)
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Leila Noorazar    , Somayeh Esmaeili , Pargol Mashati , Ahmad Gharebaghian , Sina Salari |
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| Keywords: Key words: Acute Lymphoblastic Leukemia, Apoptosis, Juniperus |
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Full-Text [PDF 788 kb]
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Type of Study: Research |
Subject:
Hematology
Published: 2020/12/30
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Full-Text: (1573 Views) |
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Sci J Iran Blood Transfus Organ 2020;17 (4): 276-286
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Investigating the apoptotic and anti-proliferative effects of Juniperus excelsa fractions on Acute Lymphoblastic leukemia cell line (Nalm-6)
Noorazar L.1, Esmaeili S.2, Mashati P.1, Gharehbaghian A.1,3, Salari S.4
1School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Traditional Medicine and Materia Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Pediatric Congenital Hematologic Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4Medical Oncology, Hematology, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Background and Objectives
Acute lymphoblastic leukemia is a hematological malignancy of lymphoid lineage . Side effects of chemotherapy drugs is a major reason for the tendency to use Herbal medicines. Previous studies on Juniperus excelsa (J.excelsa) have shown anti-cancer properties on some cancer cell lines. This study aimed to investigate the effect of three fractions of J.excelsa on acute lymphoblastic leukemia cells (Nalm-6) and normal mononuclear cells (PBMC), and find a more toxic fraction.
Materials and Methods
In this experimental study, the cytotoxic activity of these fractions in Nalm-6 was determined using Viability and MTT assay. By these tests, the more toxic fraction was selected and apoptosis was assessed and confirmed with Annexin-V/propidium iodide staining by flow cytometry. Each test was repeated at least three times. Statistical analysis was assessed by one-way ANOVA and SPSS 23.
Results
The results showed that concentrations of 2.5 and 4 µg/ml of chloroform and etherdopetrol fractions of J.excelsa, without considerable effect on PBMC, reduced significantly the cell viability and metabolic activity of Nalm-6 (p < 0.01). Also, aquae fraction had no cytotoxic effect on Nalm-6 and normal cells. The more toxic fraction, which was chloroform fraction, was selected by these tests. According to the flow cytometry test, treated Nalm-6 cells with chloroform fraction demonstrated a 13-fold increase in the number of apoptotic cells compared with control (p < 0.001).
Conclusions
Our study showed that chloroform and etherdopetrol fractions have cytotoxic and antiproliferative effects on leukemic cells (Nalm-6) and induce apoptosis.
Key words: Acute Lymphoblastic Leukemia, Apoptosis, Juniperus
Received: 6 Jul 2020
Accepted: 15 Aug 2020
Correspondence: Salari S., MD. Adult Hematologist Oncologist. Assistant Professor of Medical Oncology, Hematology, Taleghani Hospital, Shahid Beheshti University of Medical Sciences.
Postal Code: 1985711151, Tehran, Iran. Tel: (+9821) 22439982; Fax: (+9821) 22439784
E-mail: s.salari@sbmu.ac.ir |
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Noorazar L, Esmaeili S, Mashati P, Gharebaghian A, Salari S. Investigating the apoptotic and anti-proliferative effects of Juniperus excelsa fractions on Acute Lymphoblastic leukemia cell line (Nalm-6). Sci J Iran Blood Transfus Organ 2020; 17 (4) :276-286 URL: http://bloodjournal.ir/article-1-1362-en.html
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