Volume 17, Issue 2 (Summer 2020)                   Sci J Iran Blood Transfus Organ 2020, 17(2): 83-90 | Back to browse issues page

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Bahrami A, Sharifi Z, Parsania M, Haghighat S. Prevalence of anti-HBc in HBsAg negative blood donors using two enzyme immunoassays kits. Sci J Iran Blood Transfus Organ 2020; 17 (2) :83-90
URL: http://bloodjournal.ir/article-1-1316-en.html
Prevalence of anti-HBc in HBsAg negative blood donors using two enzyme immunoassays kits
A. Bahrami , Z. Sharifi * , M. Parsania , S. Haghighat
Keywords: Key words: Hepatitis B, Blood Donors, Iran
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Sci J Iran Blood Transfus Organ 2020;17 (2): 83-90
Original Article
 
 

Prevalence of anti-HBc in HBsAg negative blood donors
using two enzyme immunoassays kits
 
Bahrami A.1, Sharifi Z.2, Parsania M.3, Pourfathollah A.A.4, Haghighat S.1
 
 
1Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
 
 
Abstract
Background and Objectives
Use of kits with high sensitivity and specificity to determine the prevalence of antibodies against hepatitis B virus (anti-HBc) core antigen is important. The aim of this study was to determine the prevalence of hepatitis B virus antibody in HBsAg negative blood donors using two anti-HBc enzyme immunoassay kits (DIA.PRO and SIEMENS).
 
Materials and Methods
In this Cross-sectional study, a number of 4313 samples of HBsAg negative blood donors were collected. All samples were tested for the detection of anti-HBc (Total, IgM & IgG) by ELISA using Diapro and Siemens kits. Anti-HBc assay was performed by both kits in duplicate. Statistical analysis of data was performed using SPSS Ver. 22.0; chi-square tests were also performed.
 
Results
The study population consisted of 227 (5.3%) women and 4086 (94.7%) men. 498 (11.5%) and 384 (8.9%) samples reacted with Diapro and Siemens kits for Anti-HBc, respectively. All positive samples of both kits in duplicate test showed the same results as the previously reported results. 114 cases of reactive kits of DIA.PRO kit were negative in re-testing with SIEMENS kit. Significant differences were observed between the reactive results of the two kits (p = 0.00005).  The kappa coefficient for the reactive results of the two kits was 85%.
 
Conclusions 
Considering the significant difference between the results of the two kits, it is recommended to perform anti-HBc testing with anti-HBs and /or anti-HBe to determine the prevalence of anti-HBc in blood donors with past HBV infection.
 
Key words: Hepatitis B, Blood Donors, Iran
 
 
 
Received:  30 Oct 2019
Accepted: 29 Feb 2020
 
 

Correspondence: Sharifi Z., PhD of Virology. Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052152; Fax: (+9821) 88601555
E-mail: sharifiz@yahoo.com
Type of Study: Research | Subject: Virology
Published: 2020/06/30
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