Volume 17, Issue 2 (Summer 2020)                   Sci J Iran Blood Transfus Organ 2020, 17(2): 113-125 | Back to browse issues page

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Pazahr R, Mohammadikian M, Ali Asgari E, Mohammadi S, Rostami S, Chahardolii B, et al . Effect of curcumin and sorafenib on AKT gene expression in KG1 and U937 cell lines. Sci J Iran Blood Transfus Organ 2020; 17 (2) :113-125
URL: http://bloodjournal.ir/article-1-1297-en.html
Effect of curcumin and sorafenib on AKT gene expression in KG1 and U937 cell lines
R. Pazahr * , M. Mohammadikian , E. Ali Asgari , S. Mohammadi , Sh. Rostami , B. Chahardolii , D. Babakhani , M. Nikbakht
Keywords: Key words: Acute Myeloid Leukemia, Curcumin, Sorafenib
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Sci J Iran Blood Transfus Organ 2020; 17(2): 113-125
Original Article
 
Effect of curcumin and sorafenib on AKT gene
expression in KG1 and U937 cell lines
 
Pazahr R.1, Mohammadikian M.1, Ali Asgari E.2, Mohammadi S.1, Rostami Sh.1,
Chahardolii B.1, Babakhani D.1, Nikbakht M.1
 
 
1Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran
2Islamic Azad University South Tehran Branch, Tehran, Iran
 
 
 
Abstract
Background and Objectives
Acute myeloid leukemia is a heterozygous hematologic malignancy that is manifested by the     accumulation of hematopoietics stem cells in peripheral blood and bone marrow. Anticancer effects and  cryotoxic activity of curcumin have been proven frequently in many cancers. Sorafenib is known as an inhibitor of angiogenesis which prevent cells’ survival. In the present study, the effects of curcumin and sorafenib and their combination were evaluated on AML cells.
 
Materials and Methods
In this experimental study, U937 and KG-1 cells were treated with different concentration of curcumin and sorafenib and their combination.  MTT assay was applied to assess the viability of cells.  Percentage of apoptotic cells was evaluated by annexin PI staining. Also Real Time PCR was performed to investigte the level of  AKT mRNA expression.
 
Results
Our data showed that the percentage of apoptotic cells significantly increased in response to treatment with  curcumin (40µM in KG-1 and U937 cell lines), sorafenib (5 µM and 7 µM in U937 and KG-1, respectively) and their combination. Moreover, the mRNA level of AKT gene was downregulated in KG-1 and U937 cells.
 
Conclusions 
Our results suggest that combination of curcumin and sorafenib could lead to promote apoptosis. Furthurermore, downregulation of AKT gene shows that these agents can be considered as effective agents on AML cells. 
 
Key words: Acute Myeloid Leukemia, Curcumin, Sorafenib
 
 
 
 
 
 
Received: 13 Aug 2019
Accepted:  1 Jan  2020
 
 
Correspondence: Nikbakht  M., PhD in Medical Biotechnology. Associated Professor of Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Shariati Hospital.
Postal Code: 1411713131, Tehran, Iran. Tel: (+9821) 84902635; Fax: (+9821) 84902635
:E-mail
m-nikbakht@sina.tums.ac.ir
Type of Study: Research | Subject: Hematology and Oncology
Published: 2020/06/30
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