Volume 15, Issue 3 (Autumn 2018)                   Sci J Iran Blood Transfus Organ 2018, 15(3): 210-225 | Back to browse issues page

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Najafi D, Fathi E, Farahzadi R. Investigation of rat bone marrow mesenchymal stem cells effect on apoptosis and cell surfacof CD33 and CD34 as an approach to treatment e markers expression of chronic myeloid leukemia. Sci J Iran Blood Transfus Organ 2018; 15 (3) :210-225
URL: http://bloodjournal.ir/article-1-1181-en.html
Investigation of rat bone marrow mesenchymal stem cells effect on apoptosis and cell surfacof CD33 and CD34 as an approach to treatment e markers expression of chronic myeloid leukemia
Davood Najafi , Ezzatollah Fathi * , Raheleh Farahzadi
Keywords: Key words: Myeloid Leukemia‘Chronic, Bone Marrow Stromal Cells, Apoptosis, Co-culture
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    References:
  1. Hehlmann R, Hochhaus A, Baccarani M. Chronic myeloid leukaemia. Lancet 2007; 370(9584): 342-50.
  2. Apperley JF. Chronic myeloid leukaemia. Lancet 2015; 385(9976): 1447-59.
  3. Ailawadhi S, Akard LP, Miller CB, Jillella A, DeAngelo DJ, Ericson SG, et al. Exploratory study on the impact of switching to nilotinib in 18 patients with chronic myeloid leukemia in chronic phase with suboptimal response to imatinib. Ther Adv Hematol 2017; 8(1): 3-12.
  4. Minguell JJ, Erices A, Conget P. Mesenchymal stem cells. Exp Biol Med (Maywood) 2001; 226(6): 507-20.
  5. Ramasamy R, Lam EW, Soeiro I, Tisato V, Bonnet D, Dazzi F. Mesenchymal stem cells inhibit proliferation and apoptosis of tumor cells: impact on in vivo tumor growth. Leukemia 2007; 21(2): 304-10.
  6. Qiao L, Xu Z, Zhao T, Zhao Z, Shi M, Zhao RC, et al. Suppression of tumorigenesis by human mesenchymal stem cells in a hepatoma model. Cell Res 2008; 18(4): 500-7.
  7. Otsu K, Das S, Houser SD, Quadri SK, Bhattacharya S, Bhattacharya J. Concentration-dependent inhibition of angiogenesis by mesenchymal stem cells. Blood 2009; 113(18): 4197-205.
  8. Cousin B, Ravet E, Poglio S, De Toni F, Bertuzzi M, Lulka H, et al. Adult stromal cells derived from human adipose tissue provoke pancreatic cancer cell death both in vitro and in vivo. PLoS One 2009; 4(7):
 
e6278.
  1. Noyce RS, Bondre DG, Ha MN, Lin L-T, Sisson G, Tsao MS, et al. Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus. PLoS Pathog 2011; 7(8): e1002240.
  2. Inoue T, Swain A, Nakanishi Y, Sugiyama D. Multicolor analysis of cell surface marker of human leukemia cell lines using flow cytometry. Anticancer Res 2014; 34(8): 4539-50.
  3. Jin CH, Takada H, Nomura A, Takahata Y, Nakayama H, Kajiwara M, et al. Immunophenotypic and functional characterization of CD33+ CD34+ cells in human cord blood of preterm neonates. Exp Hematol 2000; 28(10): 1174-80.
  4. Freeman SD, Kelm S, Barber EK, Crocker PR. Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules. Blood 1995; 85(8): 2005-12.
  5. Ly JD, Grubb D, Lawen A. The mitochondrial membrane potential (deltapsi(m)) in apoptosis; an update. Apoptosis 2003; 8(2): 115-28.
  6. Lebedeva IV, Su ZZ, Sarkar D, Fisher PB. Restoring apoptosis as a strategy for cancer gene therapy: focus on p53 and mda-7. Semin Cancer Biol 2003; 13(2): 169-78.
  7. Lin LL, Huang HC, Juan HF. Revealing the molecular mechanism of gastric cancer marker annexin A4 in cancer cell proliferation using exon arrays. PLoS One 2012; 7(9): e44615.
  8. Cartier A, Komai T, Masucci MG. The Us3 protein kinase of herpes simplex virus 1 blocks apoptosis and induces phosporylation of the Bcl-2 family member Bad. Exp Cell Res 2003; 291(1): 242-50.
  9. Fathi E, Farahzadi R. Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/beta-catenin signaling pathways. PLoS One 2017; 12(3): e0173877.
  10. Farahzadi R, Fathi E, Mesbah-Namin SA, Zarghami N. Zinc sulfate contributes to promote telomere length extension via increasing telomerase gene expression, telomerase activity and change in the TERT gene promoter CpG island methylation status of human adipose-derived mesenchymal stem cells. PLoS One 2017; 12(11): e0188052.
  11. Fathi E, Farahzadi R, Charoudeh HN. L-carnitine contributes to enhancement of neurogenesis from mesenchymal stem cells through Wnt/β-catenin and PKA pathway. Exp Biol Med (Maywood) 2017; 242(5): 482-6.
  12. Trela E, Glowacki S, Błasiak J. Therapy of chronic myeloid leukemia: twilight of the imatinib era? ISRN Oncol 2014; 2014: 596483.
  13. Fathi E, Farahzadi R. Isolation, culturing, characterization and aging of adipose tissue-derived mesenchymal stem cells: a brief overview. Braz Arch Biol Technol 2016; 59: e16150383.
  14. Mobarak H, Fathi E, Farahzadi R, Zarghami N, Javanmardi S. L-carnitine significantly decreased aging of rat adipose tissue-derived mesenchymal stem cells. Vet Res Commun 2017; 41(1): 41-7.
  15. Farahzadi R, Mesbah-Namin SA, Zarghami N, Fathi E. L-carnitine Effectively Induces hTERT Gene Expression of Human Adipose Tissue-derived Mesenchymal Stem Cells Obtained from the Aged Subjects. Int J Stem Cells 2016; 9(1): 107-14.
  16. Subhashini J, Mahipal SV, Reddy MC, Reddy MM, Rachamallu A, Reddanna P. Molecular mechanisms in C-Phycocyanin induced apoptosis in human chronic myeloid leukemia cell line-K562. Biochem Pharmacol 2004; 68(3): 453-62.
  17. Xu X, Zeng Z, Yao W, Wang X, Sun D, Ka W, et al. Biomechanical alterations of dendritic cells by co-culturing with K562 CML cells and their potential role in immune escape. J Biomech 2010; 43(12): 2339-47.
  18. Han Y, Wang Y, Xu Z, Li J, Yang J, Li Y, et al. Effect of bone marrow mesenchymal stem cells from blastic phase chronic myelogenous leukemia on the growth and apoptosis of leukemia cells. Oncol Rep 2013; 30(2): 1007-13.
  19. Damiano J, Hazlehurst L, Dalton W. Cell adhesion-mediated drug resistance (CAM-DR) protects the K562 chronic myelogenous leukemia cell line from apoptosis induced by BCR/ABL inhibition, cytotoxic drugs, and [gamma]-irradiation. Leukemia 2001; 15(8): 1232-9.
  20. Tian LL, Yue W, Zhu F, Li S, Li W. Human mesenchymal stem cells play a dual role on tumor cell growth in vitro and in vivo. J Cell Physiol 2011; 226(7): 1860-7.
  21. Álvarez-Barrientos A, Arroyo J, Cantón R, Nombela C, Sánchez-Pérez M. Applications of flow cytometry to clinical microbiology. Clin Microbiol Rev 2000; 13(2): 167-95.
  22. Secchiero P, Zorzet S, Tripodo C, Corallini F, Melloni E, Caruso L, et al. Human bone marrow mesenchymal stem cells display anti-cancer activity in SCID mice bearing disseminated non-Hodgkin's lymphoma xenografts. PLoS One 2010; 5(6): e11140.
  23. Kamesaki H. Mechanisms involved in chemotherapy-induced apoptosis and their implications in cancer chemotherapy. Int J Hematol 1998; 68(1): 29-43.
  24. Ding Y, Lu H, Lu S, Lu R, Liu P, Wu Y, et al. Effects of human bone marrow mesenchymal stem cells on proliferation and apoptosis of k562 cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi 2009; 17(1): 137-40. [Article in Chinese]
  25. Zhang HM, Zhang LS. Influence of human bone marrow mesenchymal stem cells on proliferation of chronic myeloid leukemia cells. Ai Zheng 2009; 28(1): 29-32.
  26. Muroi K, Nakamurat M, Amemiya Y, Suda T, Miura Y. Expression of c-kit receptor (CD 117) and CD34 in leukemic cells. Leuk Lymphoma 1995; 16(3-4): 297-305.
  27. De Fabritiis P, Dowding C, Bungey J, Chase A, Angus G, Szydlo R, et al. Phenotypic Characterization of Normal and CML CD34-Positive Cells: Only the Most Primitive CML Progenitors Include Ph-neg Cells. Leuk Lymphoma 1999; 11(1-2): 51-61.
  28. López-Karpovitch X, Cárdenas R, Piedras J. Immunophenotypic characteristics of the blast crisis in chronic myeloid leukemia. Revista de investigacion clinica; organo del 1997; 49(1): 31-6.
  29. Pietarinen PO, Eide CA, Ayuda-Durán P, Potdar S, Kuusanmäki H, Andersson EI, et al. Differentiation status of primary chronic myeloid leukemia cells affects sensitivity to BCR-ABL1 inhibitors. Oncotarget 2017; 8(14): 22606-15.
 
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Sci J Iran Blood Transfus Organ 2018; 15(3): 210-225
Original Article
 

 

Investigation of rat bone marrow mesenchymal stem cells
effect on apoptosis and cell surface markers expression
of CD33 and CD34 as an approach to treatment
of chronic myeloid leukemia
 
Najafi D.1, Fathi E.1, Farahzadi R.2
 
1Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
2Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
 
 
Abstract
Background and Objectives
Drug resistance is one of the main challenges in the treatment of chronic myeloid leukemia (CML). In this study, with the aim of inducing apoptosis in K562 cell line (CML), this cell line was co-cultured with rat bone marrow mesenchymal stem cells (rBMSCs). Then, the rate of apoptosis as well as cell surface markers expression of CD33 and CD34 in the K562 cells line was evaluated.
 
Materials and Methods
In this experimental study, after isolating rBMSCs, the multilineag differentiation capacity of rBMSCs as well as the mesenchymal stemness of these cells was identified by evaluation of CD90, CD105, CD45, and CD56 cell surface. Then, the K562 cells were subjected for evaluation of CD33 and CD34 cell surface markers and apoptosis using a flow cytometry technique.
 
Results
Immunophenotyping of the cells indicated positive expression of CD90 and CD105 and negative expression of CD45 and CD56. The expression of CD33 and CD34 cell markers in the K562 cell line was increased 45.2% and 76.5%, respectively. Also, induction of apoptosis in the K562 cell line significantly increased (56.6%) compared to the control (p < 0.05).
 
Conclusions 
Briefly, this study showed that the co-culturing of the K562 cells line with rBMSCs causes significant increase in the expression of CD33 and CD34 cell surface markers as well as induction of apoptosis in the K562 cells (p < 0.05). This effect can be through the secreted factors and cytokines of rBMSCs; however, better evaluation in terms of type of cytokines is recommended in the future studies.
 
Key words: Myeloid Leukemia‘Chronic, Bone Marrow Stromal Cells, Apoptosis, Co-culture
 
 
 
 
 
Received: 24 Feb 2018
Accepted:  9 May 2018
 
 

Correspondence: Fathi E., Specialist in Veterinary Clinical Pathology. Associate Professor of Faculty of Veterinary Medicine, University of Tabriz.
Postal Code: 5166616471, Tabriz, Iran. Tel: (+9841) 13392351; Fax: (+9841) 13357834
E-mail:
ez.fathi@tabrizu.ac.ir
Type of Study: Research | Subject: Stem cells
Published: 2018/12/4
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