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:: Volume 9, Issue 3 And بخش 2 (Stem Cell Supplement , Autumn 2012) ::
Sci J Iran Blood Transfus Organ 2012, 9(3 And بخش 2): 239-250 Back to browse issues page
HOXB4 overexpression increases cord blood CD34+ hematopoietic stem cells population during ex vivo expansion
M. Allahbakhshian Farsani, M. Forozandeh Moghadam Dr. , N. Amirizadeh Dr., M. Soleimani Dr., A.A. Pourfatholah Dr.
Abstract:   (13447 Views)

  Abstract

 Background and Objectives

 Nowadays, cord blood hematopoietic stem cells (HSCs) are well known as a very valuable source of cells for cell therapy purposes. But unfortunately, the insufficient number of them is a limiting factor for their employment in adult bone marrow transplantation. Cord blood HSCs expansion is an approach for alleviating this problem. It has been shown by several studies during in vivo investigations in mouse models that HOXB4 is one of the most effective tools for inducing HSCs self-renewal and thus HSCs expansion. In this study, we tried to enhance self-renewal of cord blood HSCs by HOXB4 overexpression during ex vivo expansion period.

 

 Materials and Methods

 In this fundamental-applied research, HSCs were transducted by lentiviral vectors containing HOXB4. HSCs were then cultured in IMDM medium containing 10% FBS and early acting cytokines (Flt3L, SCF, Tpo) for 8 days. Thereafter, we evaluated HOXB4 expression by RT-PCR. The CD34+ subpopulation were also examined by flow cytometry.

 

 Results

 We detected a high level of HOXB4 gene expression in HOXB4 transducted HSCs relative to the control. We observed that HOXB4 over-expression dramatically increases CD34+ subpopulation of HSCs. The increase in the number of CD34+ HSCs is an indicator of HSCs self-renewal during ex vivo expansion period.

 

 Conclusions

 All in all, in this survey we observed that HOXB4 overexpression markedly increases CD34+ HSCs during ex vivo expansion period. This approach can be very effective for overcoming the limited number of cord blood HSCs for cell therapy purposes.

  

Keywords: Key words: Hematopoietic Stem Cells, HOXB4 protein, human, Lentivirus
Full-Text [PDF 721 kb]   (1923 Downloads)    
Type of Study: Research | Subject: Stem cells
Published: 2013/08/27
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Allahbakhshian Farsani M, Forozandeh Moghadam M, Amirizadeh N, Soleimani M, Pourfatholah A. HOXB4 overexpression increases cord blood CD34+ hematopoietic stem cells population during ex vivo expansion . Sci J Iran Blood Transfus Organ. 2012; 9 (3) :239-250
URL: http://bloodjournal.ir/article-1-664-en.html


Volume 9, Issue 3 And بخش 2 (Stem Cell Supplement , Autumn 2012) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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