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:: Volume 9, Issue 3 And بخش 2 (Stem Cell Supplement , Autumn 2012) ::
Sci J Iran Blood Transfus Organ 2012, 9(3 And بخش 2): 199-213 Back to browse issues page
Effect of mineralized bone allograft scaffold with coverage of UCB-derived stromal cells on ex vivo expansion of CD34+ cells
Z.S. Hashemi, M. Forouzandeh Moghadam Dr. , M. Soleimani Dr., Gh. Khamisipour Dr., M. Mossahebi Mohammadi
Abstract:   (17250 Views)

  

  Abstract

 Background and Objectives

 Bone marrow transplantation with umbilical cord blood (UCB) in adult recipients is limited mainly by a low CD34+ cell dose. To overcome this shortcoming by minimum manipulation, mineralized bone allograft (MBA) scaffold coated by UCB-USSC (unrestricted somatic stem cells) was used to expand CD34+ cells from UCB.

  

 Materials and Methods

 In this experimental study, UCB-USSCs were isolated and characterized by morphologic and immunophenotypical analysis. Then they were seeded on MBA (for 3D figuration) and culture plate (for 2D figuration) as a feeder layer. CD34+ cells were isolated from the UCB (by MACS method) and were expanded in 2D and 3D conditions for 3 weeks. At the end, cell count, flow cytometry, cologenic assay, and LTC-IC were done.

  

 Results

 After 3 weeks, ex vivo expansion of UCB-CD34+ was enhanced 250 ± 13.2 in 3D. The highest CFC expansion and LTC-IC were observed at day 14. Flow cytometry analysis showed the lowest percentage in 3D culture.

  

 Conclusions

 USSCs by 3D MBA structure produce the hematopoietic cytokine, extracellular matrix and binding molecules they also create the intercellular interaction that can be used as a suitable feeder layer. It provides an ex vivo mimicry of bone marrow niche by enhancing surface/volume ratio. This model could be a suitable and alternative environment for HSCs expansion and hematopoiesis.

  

 

Keywords: Key words : MBA, Hematopoietic Stem Cells, Umblical Cord Blood, Allograft
Full-Text [PDF 525 kb]   (2091 Downloads)    
Type of Study: Research | Subject: Stem cells
Published: 2013/08/27
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Hashemi Z, Forouzandeh Moghadam M, Soleimani M, Khamisipour G, Mossahebi Mohammadi M. Effect of mineralized bone allograft scaffold with coverage of UCB-derived stromal cells on ex vivo expansion of CD34+ cells. Sci J Iran Blood Transfus Organ. 2012; 9 (3) :199-213
URL: http://bloodjournal.ir/article-1-661-en.html


Volume 9, Issue 3 And بخش 2 (Stem Cell Supplement , Autumn 2012) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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