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:: Volume 8, Issue 3 (Autumn 2011) ::
Sci J Iran Blood Transfus Organ 2011, 8(3): 174-185 Back to browse issues page
Evaluation of the expression of self renewal genes(Oct-4, Nanog, Sox2, Nucleostemin, Bmi and Zfx) in bladder, colon and prostate cancers and in cancer cell lines (LNCaP, HepG2, HT-29, CaCo2, HT-1376)
S. Amini Dr., F. Fathi Dr. , K. Parivar Dr., B. Nikkho Dr., H. Sofimajidpour Dr., J. Mobaleghi Dr., B. Davari Dr.
Abstract:   (18764 Views)

 

  Abstract

 Background and Objectives

 Uncontrolled self renewal plays a direct function in different types of carcinoma progression. Here we examined the expression of self renewal regulatory factors such as Oct4, Nanog, Sox2, Nucleostemin, Zfx, Bmi-1 in colon, prostate, bladder and liver cancers in human samples and cancer cell lines.

 

 Materials and Methods

 We used RT-PCR to examine the expression of these genes in 10 tumors of bladder, 5 tumors of prostate, 5 tumors of colon , 5 normal tissues of colon, and cancer cell lines. The expression of Oct-4 and Nucleostemin at protein level was further determined by immunocytochemical (ICC) analysis in cancer cell lines.

 

 Results

 We designed specific primers to amplify a segment of Oct4, Nanog, Sox2, Nucleostemin, Bmi and Zfx. As expected DNA fragment of these genes based on designated primer was amplified in the PCR reaction. We detected the expression of these genes in almost all of the examined tumor samples and cancer cell lines that we used. Oct4 and Nucleostemin proteins were expressed in both nuclear and cytoplasmic in cancer cell lines. No immunoreactivity was observed in negative controls, which were incubated in the absence of primary antibody.

 

 Conclusions

 Collectively, our results indicated that in a tumor population a rare subpopulation of cells within the tumor cell mass has the potential of self renewal, and suggested that their expression can be used as potential tumor markers in diagnosis and/or prognosis of tumors. These results confirm the potential value of the cancer stem-cell theory in cancer therapy.

 

 

Keywords: Sox2 protein, human, NANOG protein, human, Zfx protein, human, Colonic Neoplasms, Prostatic Neoplasms, Unirary Bladder Neoplasms
Full-Text [PDF 618 kb]   (3348 Downloads)    
Type of Study: Research | Subject: Stem cells
Published: 2014/07/22
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Amini S, Fathi F, Parivar K, Nikkho B, Sofimajidpour H, Mobaleghi J et al . Evaluation of the expression of self renewal genes(Oct-4, Nanog, Sox2, Nucleostemin, Bmi and Zfx) in bladder, colon and prostate cancers and in cancer cell lines (LNCaP, HepG2, HT-29, CaCo2, HT-1376). Sci J Iran Blood Transfus Organ. 2011; 8 (3) :174-185
URL: http://bloodjournal.ir/article-1-515-en.html


Volume 8, Issue 3 (Autumn 2011) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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