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:: Volume 8, Issue 2 (Summer 2011) ::
Sci J Iran Blood Transfus Organ 2011, 8(2): 79-87 Back to browse issues page
In vitro differentiation of human umbilical vein mesenchymal stem cells into hepatocyte-like cells
A. R., A. Amini Dr., M. Azadbakht Dr., B. Nikkhoo Dr., M. Aboozari Dr., F. Fathi Dr.
Abstract:   (20553 Views)

  In vitro differentiation of human umbilical vein

  mesenchymal stem cells into hepatocyte-like cells

  

 

  Raufi A.1 , Amini A.1 , Azadbakht M.1 , Nikkhoo B.2,3 , Aboozari M.3 , Fathi F2,3

 

 

  1 Razi University, Kermanshah, Iran

  2 Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran

  3 Kurdistan University of Medical Sciences, Sanandaj, Iran

 

  

  Abstract

 Background and Objectives

 Umbilical vein mesenchymal stem cells (UVMSC) which are multipotent precursors have been recently isolated. They are capable of differentiating into various cell types including neural and cardiac cells. In this study, the differentiation ability of human UVMSC into hepatocyte cells has investigated with concentration on hepatic genes expression profile.

 

 Materials and Methods

 The UVMSC were isolated and cultured in differentiation medium containing hepatocyte growth factor (HGF) and oncostatin M (OSM) using two step protocol. Differentiation of UVMSCs into cells expressing liver-specific genes was investigated by RT-PCR and immunocytochemistry.

 

 Results

 The cells showed the remarkable transition from bipolar fibroblast-like morphology to epithelial and polygonal shapes. The temporal gene expression pattern of hepatocyte-specific genes such as CK8, CK18, HNF3β, c-Met, AFP, TTR, G6P, Transthyretin, and ALB were detected during differentiation. The immunoflourescent analysis also showed that the differentiated cells were stained positively for CK-18 protein.

 

 Conclusions

 Findings indicate that UVMSC has a potential for differentiation into the hepatic like cells.

  

 Key words : Umbilical Veins, Mesenchymal Stem Cells, Hepatocyte Growth Factor, Oncostatin M 

 Sci J Iran Blood Transfus Org 2011 8(2): 79-87

  

  

  

  

  

  

  

  Received: 29 Nov 2010

  Accepted: 15 Feb 2011

  

 

 


  Correspondence: Fathi F., PhD of Anatomy. Associate Professor, Cellular and Molecular Research Center, Kurdistan University of Medical Sciences.

  P.O.Box: 66177-13446, Sanandaj, Iran. Tel: (+98871) 6661830 Fax : (+98871) 6660051

  E-mail:

  1- Acridine Orange

  1- Biological safety cabinet

  1- Platelet Concentrate

  2- Food and Drug Administration

  3- Normal Skin Flora

  4- Platelet Rich Plasma-Platelet Concentrate

  5- Eosin-Methylene blue

  6- Thioglycolate

farfath@gmail.com

Keywords: Umbilical Veins, Mesenchymal Stem Cells, Hepatocytes, Hepatocyte Gowth Fctor, Oncostatin M
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Type of Study: Research | Subject: Stem cells
Published: 2013/08/28
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R. A, Amini A, Azadbakht M, Nikkhoo B, Aboozari M, Fathi F. In vitro differentiation of human umbilical vein mesenchymal stem cells into hepatocyte-like cells. Sci J Iran Blood Transfus Organ. 2011; 8 (2) :79-87
URL: http://bloodjournal.ir/article-1-481-en.html


Volume 8, Issue 2 (Summer 2011) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
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