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:: Volume 6, Issue 4 (Winter 2010) ::
Sci J Iran Blood Transfus Organ 2010, 6(4): 248-256 Back to browse issues page
Prevalence of hepatitis B virus genotypes to be determined by sequencing in blood donors in Kerman, Esfahan and Yazd
Z. Sharifi Dr., A. Gharehbaghian Dr. , M. Noroozi
Abstract:   (16862 Views)

  Abstract

 Background and Objectives

 Hepatitis B is one of the major diseases that has the potential to be transmitted by blood transfusion. Specific genotypes of hepatitis B virus are increasingly recognized for their clinical significance and association with particular viral mutations. The aim of this study was to investigate the prevalence of hepatitis B virus genotypes in blood donors of Kerman, Yazd and Isfehan Blood Centers.

 

 Materials and Methods

 Sera of 120 blood donors, who were positive for hepatitis B surface antigen (HBsAg) using ELISA method, were selected. HBV DNA was extracted from sera using commercial kits and the p gene sequences were amplified by nested-PCR. HBV genotypes were determined by direct sequencing of the polymerase gene of HBV. The obtained sequences were aligned with Clustal W programme. Phylogenetic trees were constructed by the Neighbor-joining (NJ) method. The data were analyzed with Fisher test by SPSS 11.5

 

 Results

 Out of 120 HBsAg positive volunteer blood donors, 69 (57.5% , CI 95% = 48.65-66.34)) were positive for HBV DNA sequence. Sequence analysis was done on the positive samples. Genotype D was detected among 100% of HBV infected volunteer blood donors.

 

 Conclusions

 The prevalence rate of genotype D was found to be 100%. These findings may have an impact on the immunological and genetic diagnosis of HBV as well as on the selection of diagnostic kits and viral quality control panels.

 

 Key words : Hepatitis B virus, Blood Donors, Genotype

 

Keywords: Hepatitis B virus, Blood Donors, Genotype
Full-Text [PDF 248 kb]   (3065 Downloads)    
Type of Study: Research | Subject: Infectious disease
Published: 2014/08/17
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Sharifi Z, Gharehbaghian A, Noroozi M. Prevalence of hepatitis B virus genotypes to be determined by sequencing in blood donors in Kerman, Esfahan and Yazd . Sci J Iran Blood Transfus Organ. 2010; 6 (4) :248-256
URL: http://bloodjournal.ir/article-1-366-en.html


Volume 6, Issue 4 (Winter 2010) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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