Scientific Journal of

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  Abstract

 Background and Objectives

 One of the main steps in reducing bacterial contamination of blood and blood components is the correct use of appropriate disinfectants at blood collection sites and blood processing areas.Virkon is one of the most effective disinfectants consisting of a high percentage of surfactant.The purpose of the present study was to test the bactericidal effect of Virkon on native species of bacteria seen in Iran by selecting a suitable concentration of Virkon.

 Materials and Methods

 This was an interventional study. 169 samples were taken from laboratory benches, instruments and outer surfaces of blood bags. After the growing bacteria were identified, their CFU/ml was also determined. Later, all the laboratory benches and instruments involved in the preparation of platelet components were disinfected using (1%) Virkon solution. 101 samples were taken from disinfected areas and swabs were plated on to standard bacteriological media and plates were read. 1100 segments from platelet bags were also separated and the platelet contents were plated and any bacterial growth were assessed using quality control department guidelines. Finally, all the data were analyzed using SPSS, Chi-square and Fischer exact test.

 Results

 Out of 169 samples which were plated before disinfection by Virkon, 96 were 56.8% gram positive b., 101 were 59.8% gram positive cocci, 159 were 94.8% gram negative b., and 139 were 82.2% gram negative cocci. Out of 101 samples post-disinfected by virkon solution, 25 came out to be 24.8% gram positive b., 17 were 16.8% gram positive cocci, and 6 were 5.9% gram negative b. Out of 1100 segments separated from platelet bags, 4 showed bacterial growth (0.0036%), 2 had gram positive b. growth, and 2 had staph.

 Conclusions

 By using correct concentration of Virkon solution and following the exact manufacture's instruction for use, we were able to observe log reduction in bacterial contamination of the areas where blood components were prepared. The reduction of bacterial contamination in platelet components prepared after disinfection of the working areas by Virkon solution should be emphasized.