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:: Volume 2, Issue 3 (Spring 2005) ::
Sci J Iran Blood Transfus Organ 2005, 2(3): 53-58 Back to browse issues page
Preparation of heme control for cellulose acetate electrophoresis
L. Hosseini Gohari , E. Noormohammadi, Z. Attarchi
Abstract:   (33403 Views)

  Abstract

  

 Background and Objectives

  To avoid laboratory errors in the detection of hemoglobinopathies, a control sample containing hemoglobin (Hb) A,F,S or C should run with each set of samples.The aim of this project was to prepare a lyophilized heme control for the cellulose acetate electrophoresis.

  

  Materials and Methods 

  After lysing the red blood cells, the hemolysate was centrifuged at 25000 rpm. The clear red supernatant was diluted with drabkin reagent to 20-30 gr/L and Hb was converted to cyanomethemoglobin. After adding sucrose and preservatives, the hemolysate was passed through milipore filter (0.45 μ m) and then aliqouted and lyophilized.

 

 Results

 In this project three heme control samples were prepared. The first sample contains Hbs A,F,D or G ,the second one contains Hbs A,S, and the third contains Hb A,F. Although the amount of potassium cyanide used is 10 times less than other methods , the stability is more than 6 months at 4oC moreover, and the electrophoretic patterns have good resolution and the obtained CV and SD show good reproducibility .

  

 Conclusions

  The preparation method in this project is simple, reliable, cost effective and in comparison with other methods has less amount of potassium cyanide therefore, it is safe for the laboratory staff.

  

  

 Key words: Hemoglobin , Cellulose acetate, Electrophoresis, Hemoglobinopathy

Keywords: Hemoglobin, Cellulose acetate, Electrophoresis, Hemoglobinopathy
Full-Text [PDF 241 kb]   (2633 Downloads)    
Type of Study: Research | Subject: General
Published: 2014/06/30
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Hosseini Gohari L, Noormohammadi E, Attarchi Z. Preparation of heme control for cellulose acetate electrophoresis. Sci J Iran Blood Transfus Organ. 2005; 2 (3) :53-58
URL: http://bloodjournal.ir/article-1-26-en.html


Volume 2, Issue 3 (Spring 2005) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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