Volume 5, Issue 1 (spring 2008)                   Sci J Iran Blood Transfus Organ 2008, 5(1): 9-15 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bagheri N, Mortazavi Y, Ghafari H, Alimoghadam K, pourfatoullah A, Shayan N et al . Analysis of telomere length changes in chronic and blastic phases of chronic myelogenous leukemia. Sci J Iran Blood Transfus Organ 2008; 5 (1) :9-15
URL: http://bloodjournal.ir/article-1-218-en.html
Abstract:   (29854 Views)

  Abstract

 Background and Objectives

 In the mammalian cells, there is a relationship between telomere length and both cancer and senescence. Progressive telomere shortening has a role in genomic instability and has been reported in a wide range of human cancers as well as in transformation and progression to hematologic malignancies. Chronic myelogenous leukemia (CML) has different stages in the process of its progression. In this study, we examined the telomere length changes in peripheral blood leukocytes of CML patients in chronic (CP) and blastic phases (BP).

 

 Materials and Methods

 In this descriptive study, we examined the telomere length in 21 CML patients (14 in chronic and 7 in blastic phases) having reffered to Hematology–Oncology and BMT Research Center of Shariati Hospital since March 2004 using Southern blot analysis the results were then compared with age-adjusted normal controls. Data were analyzed through logestic regression and Anova.

 

 Results

 At the time of diagnosis, 71.43% of chronic phase patients had a shortened TRF compared to normal age-adjusted individuals. The mean telomere length values in chronic and blastic phases were 6.98±1.26 kb and 4.81±1.06 kb, respectively it showed significant telomere length reduction in age-adjusted normal controls. Moreover, the mean telomere length values in BP-CML showed significant statistical differences as compared to CP-CML. Mean values of telomere length reduction in CP-CML and BP-CML as compared with normal age-adjusted control group were 3.31±1.38 kb and 5.27±0.9 kb, respectively.

 

 Conclusions

 The significant statistical differences in mean telomere length of CP-CML and BP-CML as compared with age-adjusted normal controls and the apparent differences of TRF in chronic and blastic phases can be useful in prediction of phase of disease progression.

 

 Key words : Chronic myelogenous leukemia, Telomere, Southern blot

 

Full-Text [PDF 259 kb]   (3230 Downloads)    
Type of Study: Research | Subject: Hematology
Published: 2014/08/17

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Scientific Journal of Iran Blood Transfus Organ

Designed & Developed by : Yektaweb