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:: Volume 4, Issue 4 (winter 2008) ::
Sci J Iran Blood Transfus Organ 2008, 4(4): 239-246 Back to browse issues page
Globin chain synthesis for differential diagnosis of β thalassemia from α thalassemia carriers
SH. Khatami , S. Rouhi Dehboneh, S. Sadeghi, P. Saeidi, R. Mirzazadeh, P. Bayat, A. Amirkhani, A. Samavat, S. Zeinali, M.T. Akbari, H. Najmabadi
Abstract:   (24715 Views)

  Abstract

 Background and Objectives

 Globin chain synthesis and DNA analysis are among complementary tests for thalassemia diagnosis. Nowadays, DNA analysis is the only definitive method for diagnosis of suspected carriers. Despite the complexity of this heterogenic disease which is attributed to mutations in gene regulation sites or unknown mutations, globin chain synthesis has maintained its significant role in identifying different kinds of thalassemia. As a result, besides the routine application of this method , we decided to determine the ranges of α/β ratio values in different kinds of thalassemia.

 

 Materials and Methods

 In this experimental study 214 cases were divided into the control (51 cases), minor β thalassemia (24 cases), mild α (α thal. 2) thalassemia (68 cases), severe α (α thal. 1) thalassemia (44 cases), Hemoglobin H disease (6 cases), silent β ( type II) thalassemia (14 cases), δβ thalassemia (5 cases), and αδβ thalassemia (2 cases) groups. CBC, hemoglobin electrophoresis using acetate cellulose paper in alkaline pH, hemoglobin A2 measurement by column chromatography, reticulocytes percentage, hemoglobin H, RBC morphology, and globin chain synthesis were performed on each group.

 

 Results

 Significant differences were observed in mean values of RBC, hemoglobin, hematocrite, MCV, MCH, MCHC, α/β ratio in α and β thalassemia cases as compared with the control group. High prevalence of α thalassemia was observed among suspected individuals (55.2% of different kinds of α thalassemia vs. 9.8% of different kinds of atypic β thalassemia) as compared with atypic β thalassemia.

 

 Conclusions

 The mean value of α/β ratio achieved in this study was similar to the others, but with a greater standard deviation. Because of this, there exists a wider range of α/β ratio. This width of range made overlaps in different and adjacent groups. Therefore, α/β ratio cannot be used by itself to firmly diagnose the type of thalassemia. As a result, for accurate diagnosis to be made, besides considering patient's ethnicity and clinical features, it is necessary to assess the results of CBC, hemoglobin electrophoresis pattern analysis, globin chain synthesis, familial tests, and DNA analysis.

 

 Key words : β Thalassemia, α thalassemia, Globin

Keywords: β Thalassemia, α thalassemia, Globin
Full-Text [PDF 397 kb]   (4646 Downloads)    
Type of Study: Research | Subject: Hematology
Published: 2014/08/17
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Khatami S, Rouhi Dehboneh S, Sadeghi S, Saeidi P, Mirzazadeh R, Bayat P, et al . Globin chain synthesis for differential diagnosis of β thalassemia from α thalassemia carriers. Sci J Iran Blood Transfus Organ. 2008; 4 (4) :239-246
URL: http://bloodjournal.ir/article-1-187-en.html


Volume 4, Issue 4 (winter 2008) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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