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Sci J Iran Blood Transfus Organ 2007, 4(3): 205-213 Back to browse issues page
In vitro evaluation of P-Selectin expression of platelet and platelet aggregation in stored platelet concentrates
A. Sadri, A. Gharehbaghian, Sh. Vaeli, F. Asgari pour, M. Karbasizadeh, J. Ghaledi, M. Aghayeepour, T. Shashaani, A.S. Tabatabaeeian, F. Kami, M. Ahmadinejad
Abstract:   (13666 Views)

  Abstract

 Background and Objectives

 Platelets have a major role in haemostasis. Platelet concentrates are used in thrombocytopeny and platelet dysfunction disorders. Due to recent studies reporting loss of in vivo function of platelets in platelet concentrate units, we determined the level of surface expression of P-Selectin, conducted platelet aggregation tests and pH measurment to evaluate platelet activation during storage.

 

 Materials and Methods

 In a cross-sectional study, 100 platelet concentrate units prepared by PRP method were evaluated. 34, 33 and 33 units were taken from the first, second, and third day of storage respectively. For each platelet concentrate unit, pH, CD62P-Selectin and platelet aggregation tests with Arashidonic acid and Ristocetin agonists were conducted.

 

 Results

 Expression of P-Selectin in third day was significantly higher than first day (p<0.05). Platelet aggregation tests showed significant decrease in third day compared to first day (p<0.05). pH in third day was significantly higher than first day (p<0.05).

 

 Conclusions

 The present study shows that activated platelets during 3 days of storage have increased P- Selectin on their surface. Therefore, the P-Selectin expression can be used as an in vivo marker for platelet activation function.

 

 Key words : Platelet , P-Selectin, Flowcytometery, Platelet aggregation

 

Keywords: Platelet, P-Selectin, Flowcytometery, Platelet aggregation
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Type of Study: Research | Subject: Hematology
Published: 2014/08/17
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Sadri A, Gharehbaghian A, Vaeli S, Asgari pour F, Karbasizadeh M, Ghaledi J, et al . In vitro evaluation of P-Selectin expression of platelet and platelet aggregation in stored platelet concentrates. Sci J Iran Blood Transfus Organ. 2007; 4 (3) :205-213
URL: http://bloodjournal.ir/article-1-175-en.html


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Volume 4, Issue 3 (Autumn 2007) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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