[Home ] [Archive]   [ فارسی ]  
:: Main :: About us :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 18, Issue 3 (Autumn 2021) ::
Sci J Iran Blood Transfus Organ 2021, 18(3): 161-170 Back to browse issues page
The effect of sCD40L derived from platelet concentrate on cell survival and activation of Daudi cells
A. Nemati, F. Yari , M. Mohammadipour, N. Rezaei
Abstract:   (187 Views)

Abstract

Background and Objectives

Previous studies have suggested an association between the importance of CD40L and B lymphocytes. CD40L derived from platelets plays an important role in the activation of B lymphocytes and humoral immunity. The aim of the present study was to investigate the effect of purified CD40L from platelet product on the survival and activation of Daudi cells as an available surrogate cell line for peripheral blood B lymphocytes.

 

Materials and Methods

In an experimental study, Purified CD40L from platelet product was exposed to Daudi cells at concentrations of 500 and 1000 ng/mL. After 48 and 72 hours of exposure, cell survival was studied with MTT assay and CD27 expression with flow cytometry. SPSS software and Independent T Test and Mann-Whitney tests were used for statistical analysis.

 

Results

The results showed a significant increase in cell survival at 48 hours after exposure to 1000 ng/mL of CD40L with p = 0.04 (0.61 ± 0.41) compared to the control group with the mean of 0.30 ± 0.15 while in 500 ng/mL with (0.58 ± 0.35), it was insignificant. CD27 expression did not show a significant increase in all concentrations.

 Conclusions 

Soluble CD40L has been effective in the survival of Daudi cells at higher concentrations. A slight increase in CD27 as one of the activation markers of B lymphocytes suggests the need to provide more appropriate conditions such as the use of cytokines in the cell culture medium.

Keywords: Platelets, Survival, B-Lymphocytes
ت
Full-Text [PDF 503 kb]   (57 Downloads) |   |   Full-Text (HTML)  (58 Views)  
Type of Study: Research | Subject: Imunology
Published: 2021/10/2
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nemati A, Yari F, Mohammadipour M, Rezaei N. The effect of sCD40L derived from platelet concentrate on cell survival and activation of Daudi cells. Sci J Iran Blood Transfus Organ. 2021; 18 (3) :161-170
URL: http://bloodjournal.ir/article-1-1378-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 3 (Autumn 2021) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 4343