Volume 12, Issue 3 (Autumn 2015)                   Sci J Iran Blood Transfus Organ 2015, 12(3): 266-276 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rafieemehr H, Kheirandish M, Soleimani M. Isolation, expansion, and in vitro differentiation of human umbilical cord blood mesenchymal stromal cells into neural progenitor cells. Sci J Iran Blood Transfus Organ 2015; 12 (3) :266-276
URL: http://bloodjournal.ir/article-1-937-en.html
Full-Text [PDF 394 kb]   (2557 Downloads)     |   Abstract (HTML)  (6464 Views)
Full-Text:   (3748 Views)
References :  
  1. Etemadifar M, Sajjadi S, Nasr Z, Firoozeei TS, Abtahi S-H, Akbari M, et al. Epidemiology of multiple sclerosis in Iran: a systematic review. Eur Neurol 2013; 70(5-6): 356-63.
  2. Kingwell E, Marriott JJ, Jetté N, Pringsheim T, Makhani N, Morrow SA, et al. Incidence and prevalence of multiple sclerosis in Europe: a systematic review. BMC Neurol 2013; 13(1): 128.
  3. Milo R, Miller A. Revised diagnostic criteria of multiple sclerosis. Autoimmun Rev 2014; 13(4-5): 518-24.
  4. Liu S, Li C, Xing Y, Tao F. Effect of transplantation of human embryonic stem cell-derived neural progenitor cells on adult neurogenesis in aged hippocampus. Am J Stem Cells 2014; 3(1): 21-6.
  5. Joyce N, Annett G, Wirthlin L, Olson S, Bauer G, Nolta JA. Mesenchymal stem cells for the treatment of neurodegenerative disease. Regen Med 2010; 5(6): 933-46.
  6. Tisato V, Naresh K, Girdlestone J, Navarrete C, Dazzi F. Mesenchymal stem cells of cord blood origin are effective at preventing but not treating graft-versus-host disease. Leukemia 2007; 21(9): 1992-9.
 
 
 
 
 
  1. Zhang J, Li Y, Chen J, Cui Y, Lu M, Elias SB, et al. Human bone marrow stromal cell treatment improves neurological functional recovery in EAE mice. Exp Neurol 2005; 195(1): 16-26.
  2. Hoveizi E, Tavakol S, Ebrahimi-Barough S. Neuroprotective Effect of Transplanted Neural Precursors Embedded on PLA/CS Scaffold in an Animal Model of Multiple Sclerosis. Mol Neurobiol 2015: 51(3): 1334-42.
  3. Harris VK, Yan QJ, Vyshkina T, Sahabi S, Liu X, Sadiq SA. Clinical and pathological effects of intrathecal injection of mesenchymal stem cell-derived neural progenitors in an experimental model of multiple sclerosis. J Neurol Sci 2012; 313(1-2): 167-77.
  4. Harris VK, Faroqui R, Vyshkina T, Sadiq SA. Characterization of autologous mesenchymal stem cell-derived neural progenitors as a feasible source of stem cells for central nervous system applications in multiple sclerosis. Stem Cells Transl Med 2012; 1(7): 536-47.
  5. Woodbury D, Schwarz EJ, Prockop DJ, Black IB. Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res 2000; 61(4): 364-70.
  6. Sanchez-Ramos J, Song S, Cardozo-Pelaez F, Hazzi C, Stedeford T, Willing A, et al. Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp Neurol 2000; 164(2): 247-56.
  7. Mezey E, Chandross KJ, Harta G, Maki RA, McKercher SR. Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow. Science 2000; 290(5497): 1779-82.
  8. Brazelton TR, Rossi FM, Keshet GI, Blau HM. From marrow to brain: expression of neuronal phenotypes in adult mice. Science 2000; 290(5497): 1775-9.
  9. Weimann JM, Johansson CB, Trejo A, Blau HM. Stable reprogrammed heterokaryons form spontaneously in Purkinje neurons after bone marrow transplant. Nat Cell Biol 2003; 5(11): 959-66.
  10. Wagers AJ, Sherwood RI, Christensen JL, Weissman IL. Little evidence for developmental plasticity of adult hematopoietic stem cells. Science 2002; 297(5590): 2256-9.
  11. Morshead CM, Benveniste P, Iscove NN, van der Kooy D. Hematopoietic competence is a rare property of neural stem cells that may depend on genetic and epigenetic alterations. Nat Med 2002; 8(3): 268-73.
  12. Kögler G, Sensken S, Wernet P. Comparative generation and characterization of pluripotent unrestricted somatic stem cells with mesenchymal stem cells from human cord blood. Exp Hematol 2006; 34(11): 1589-95.
  13. Kögler G, Somville T, Göbel U, Hakenberg P, Knipper A, Fischer J, et al. Haematopoietic transplant potential of unrelated and related cord blood: the first six years of the eurocord/netcord Bank Germany. Klin Pädiatr 1999; 211(04): 224-32.
  14. Tio M, Tan KH, Lee W, Wang TT, Udolph G. Roles of db-cAMP, IBMX and RA in aspects of neural
    differentiation of cord blood derived mesenchymal-like stem cells. PLoS One 2010; 5(2): e9398.
  15. Wang T, Tio M, Lee W, Beerheide W, Udolph G. Neural differentiation of mesenchymal-like stem cells from cord blood is mediated by PKA. Biochem Biophys Res Commun 2007; 357(4): 1021-7.
  16. Deng W, Obrocka M, Fischer I, Prockop DJ. In Vitro Differentiation of Human Marrow Stromal Cells into Early Progenitors of Neural Cells by Conditions That Increase Intracellular Cyclic AMP. Biochem Biophys Res Commun 2001; 282(1): 148-52.
  17. Hou L, Cao H, Wang D, Wei G, Bai C, Zhang Y, et al. Induction of umbilical cord blood mesenchymal stem cells into neuron-like cells in vitro. Int J Hematol 2003; 78(3): 256-61.
  18. Lee OK, Kuo TK, Chen WM, Lee KD, Hsieh SL, Chen TH. Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood 2004; 103(5): 1669-75.
  19. Tropel P, Platet N, Platel JC, Noel D, Albrieux M, Benabid AL, et al. Functional neuronal differentiation of bone marrow-derived mesenchymal stem cells. Stem Cells 2006; 24(12): 2868-76.
  20. Nemati Sh. Differentiation of human bone marrow mesenchymal stem cells to neural-like cells in vitro. Tehran University Medical Journal 2009; 67: 527-34. [Article in Farsi]
  21. Kashafi E, Karimi Jashni H, Erfaniyan S, Solhjou K, Sepidkar A, Fakhryniya H. Transdifferentiation of human synovium-derived mesenchymal stem cell into neuronal like cells in vitro. Pars Journal of Medical Sciences 2013; 11(2): 39-49.
  22. Taran R, Mamidi MK, Singh G, Dutta S, Parhar IS, John JP, et al. In vitro and in vivo neurogenic potential of mesenchymal stem cells isolated from different sources. J Biosci 2014; 39(1): 157-69.
 
 
 
 
 
 


 
 
Original  Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2015; 12(3): 266-276
 
 

Isolation, expansion, and in vitro differentiation
of human umbilical cord blood mesenchymal stromal
cells into neural progenitor cells
 
 Rafieemehr H.1,2, Kheirandish M.1, Soleimani M.3
 
1Blood Transfusion Research Center,High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2School of Alliel Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
 
Abstract
Background and Objectives
Umbilical cord blood, as a source of mesenchymal stromal cells, has many advantages compared to other sources. This study aimed to provide a new method for the in vitro neural differentiation of human umbilical cord blood derived mesenchymal stromal cells (MSCs hUCB).  
 
Materials and Methods
In this experimental study, MSCs hUCBs were isolated and characterized by morphologic, adipogenic, and osteogenic differentiation and immunophenotypical analysis. Then, neural induction of MSCs hUCB was performed by using RA, bFGF, NGF, EGF, AsA , IBMX, and neurobasal medium. Then, the relative expression of neural-specific genes was investigated by quantitative real-time PCR assays, REST 2009, and SPSS 11.5 software.
 
Results
Our results showed the osteocytic  and  adipocytic differentiation capacity and fibroblast-like morphology  of MSCs hUCB. Flow cytometry analysis of MSCs hUCB revealed that the cells are positive for CD105 (78%), CD73 (78%), and negative for CD45 (2%), HLA-DR (2.5%). The cells showed the remarkable transition from fibroblast-like morphology to nueral  progenitor cells. The results showed that the expression of GFAP, MBP, nestin, MAP-2 genes after neural induction significantly increased in comparison with that of the control as measured by quantitative real-time PCR assays (p < 0.05).
 
Conclusions
Treatment of MSCs hUCB with a set of growth factors and chemical materials induces neural differentiation and increases the efficiency of cell-based therapy for neurodegenerative diseases in the future. Although, the functionality of neural progenitor cells must be carefully assessed in animal models prior to use in clinical application.
 
Key words:  Mesenchymal Stromal Cells, Cell- and Tissue-Based Therapy, Cell Differentiation
 
 
 
 
Received:  7  Feb 2015
Accepted:28 Apr 2015
 
 
 

Correspondence: Kheirandish M., PhD of Immunology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box:14665-1157, Tehran, Iran. Tel: (+9821) 82052208; Fax : (+9821) 88601599
E-mail:
m.kheirandish@ibto.ir
Type of Study: Research | Subject: Stem cells
Published: 2015/09/2

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Scientific Journal of Iran Blood Transfus Organ

Designed & Developed by : Yektaweb