Volume 12, Issue 3 (Autumn 2015)                   Sci J Iran Blood Transfus Organ 2015, 12(3): 266-276 | Back to browse issues page

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Rafieemehr H, Kheirandish M, Soleimani M. Isolation, expansion, and in vitro differentiation of human umbilical cord blood mesenchymal stromal cells into neural progenitor cells. Sci J Iran Blood Transfus Organ 2015; 12 (3) :266-276
URL: http://bloodjournal.ir/article-1-937-en.html
Isolation, expansion, and in vitro differentiation of human umbilical cord blood mesenchymal stromal cells into neural progenitor cells
H. Rafieemehr , M. Kheirandish * , M. Soleimani
Keywords: Key words : Mesenchymal Stromal Cells, Cell- and Tissue-Based Therapy, Cell Differentiation
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Original  Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2015; 12(3): 266-276
 
 

Isolation, expansion, and in vitro differentiation
of human umbilical cord blood mesenchymal stromal
cells into neural progenitor cells
 
 Rafieemehr H.1,2, Kheirandish M.1, Soleimani M.3
 
1Blood Transfusion Research Center,High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2School of Alliel Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
 
Abstract
Background and Objectives
Umbilical cord blood, as a source of mesenchymal stromal cells, has many advantages compared to other sources. This study aimed to provide a new method for the in vitro neural differentiation of human umbilical cord blood derived mesenchymal stromal cells (MSCs hUCB).  
 
Materials and Methods
In this experimental study, MSCs hUCBs were isolated and characterized by morphologic, adipogenic, and osteogenic differentiation and immunophenotypical analysis. Then, neural induction of MSCs hUCB was performed by using RA, bFGF, NGF, EGF, AsA , IBMX, and neurobasal medium. Then, the relative expression of neural-specific genes was investigated by quantitative real-time PCR assays, REST 2009, and SPSS 11.5 software.
 
Results
Our results showed the osteocytic  and  adipocytic differentiation capacity and fibroblast-like morphology  of MSCs hUCB. Flow cytometry analysis of MSCs hUCB revealed that the cells are positive for CD105 (78%), CD73 (78%), and negative for CD45 (2%), HLA-DR (2.5%). The cells showed the remarkable transition from fibroblast-like morphology to nueral  progenitor cells. The results showed that the expression of GFAP, MBP, nestin, MAP-2 genes after neural induction significantly increased in comparison with that of the control as measured by quantitative real-time PCR assays (p < 0.05).
 
Conclusions
Treatment of MSCs hUCB with a set of growth factors and chemical materials induces neural differentiation and increases the efficiency of cell-based therapy for neurodegenerative diseases in the future. Although, the functionality of neural progenitor cells must be carefully assessed in animal models prior to use in clinical application.
 
Key words:  Mesenchymal Stromal Cells, Cell- and Tissue-Based Therapy, Cell Differentiation
 
 
 
 
Received:  7  Feb 2015
Accepted:28 Apr 2015
 
 
 

Correspondence: Kheirandish M., PhD of Immunology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box:14665-1157, Tehran, Iran. Tel: (+9821) 82052208; Fax : (+9821) 88601599
E-mail:
m.kheirandish@ibto.ir
Type of Study: Research | Subject: Stem cells
Published: 2015/09/2
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