Volume 12, Issue 3 (Autumn 2015)                   Sci J Iran Blood Transfus Organ 2015, 12(3): 223-232 | Back to browse issues page

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Fallah P, Timori Naghadeh H, Soleimani M, Amirizadeh N, Poopak B, Toogeh G, et al . The assessment of miR-150 and miR-155 on chronic myelogenous leukemia patients. Sci J Iran Blood Transfus Organ 2015; 12 (3) :223-232
URL: http://bloodjournal.ir/article-1-906-en.html
The assessment of miR-150 and miR-155 on chronic myelogenous leukemia patients
P. Fallah , H. Timori Naghadeh * , M. Soleimani , N. Amirizadeh , B. Poopak , Gh. Toogeh , E. Arefian , Sh. Bolouri , T. Golkar , Z. Pirmohammad Jamaat
Keywords: Key words : MicroRNAs, miR-150 ٬ human, miR-155 ٬ human, Leukemia ٬ Chronic Myelogenous
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Sci J Iran Blood Transfus Organ 2015; 12(3): 223-232
 
Original  Article
 
 

The assessment of miR-150 and miR-155 on chronic
myelogenous leukemia patients
 
Fallah P.1,2, Timori Naghadeh H.1, Soleimani M.3, Amirizadeh N.1, Poopak B.4, Toogeh Gh.5,
Arefian E.6, Bolouri Sh.7, Golkar T.7, Pirmohammad Jamaat Z.1
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Allied-Health Faculty, Alborz University of Medical Sciences, Karaj, Iran
3Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4School of Allied Medicine, Medical Branch of Islamic Azad University, Tehran, Iran 
5School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
6Biology School of Tehran University, Tehran, Iran
7Payvandlab Clinical & Speciality Laboratory, Tehran, Iran
 
Abstract
Background and Objectives
Micro RNAs are a class of small non-coding RNAs which have been recently shown to play a crucial role in major cellular processes such as development and differentiation through post-transcriptional regulation. The correlation of microRNA expression levels with the pathogenesis of some hematologic malignancies including acute myeloid leukemia is already known. The aim of this study was to investigate the expression of mir-150 and mir-155 in chronic myelogenous leukemia patients. miRNA expression level changes can be used for diagnosis, treatment and estimation of Minimal Residual Disease (MRD).
 
Materials and Methods
In this descriptive study, the expression of miR-150 and miR-155 in 25 newly diagnosed CML patients in chronic phase and 25 healthy subjects as the control all in Payvand Medical Laboratory were examined by Stem-loop RT-PCR and Real Time PCR techniques. For data analysis, Pearson correlation analysis was used.
 
Results
miR-150 and miR-155 were down-regulated in CML patients rather than in healthy subjects (0.512 and 0.2552, respectively). There was no correlation between the laboratory findings and the miR expression level. But miR-155 was inversely associated with age (p ≤  0.01).
 
Conclusions
In conclusion, the decreasing expression of miR-150 and miR-155 in chronic myelogenous leukemia is indicative of their induced expression in suppressing proliferation of cells in this disease. Other studies are also required to elucidate the exact role of microRNA by identifying their target genes.
 
Key words: MicroRNAs, miR-150٬ human, miR-155٬ human, Leukemia٬ Chronic Myelogenous
 
Received: 15  Oct 2014
Accepted: 23 Jan 2015
 
 
 

Correspondence: Timori Naghadeh H., Pathologist. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 1157-14665, Tehran, Iran. Tel: (+9821) 88601564; Fax: (+9821) 88060717
E-mail: timori13@gmail.com
Correspondence: Soleimani M., PhD of Hematology. Associate Professor of Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University.
P.O.Box: 14115-111, Tehran, Iran. Tel: (+9821) 88011001; Fax: (+9821) 88013030
E-mail: soleim_m@modares.ac.ir
Type of Study: Research | Subject: Hematology
Published: 2015/09/2
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