Volume 12, Issue 3 (Autumn 2015)                   Sci J Iran Blood Transfus Organ 2015, 12(3): 277-286 | Back to browse issues page

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Moghaddam F, Oodi A, Nikougoftar Zarif M, Amirizadeh N. Evaluation of platelet microparticles effect on the expression of CXCR4 in umbilical cord blood derived CD133+ cells. Sci J Iran Blood Transfus Organ 2015; 12 (3) :277-286
URL: http://bloodjournal.ir/article-1-893-en.html
Evaluation of platelet microparticles effect on the expression of CXCR4 in umbilical cord blood derived CD133+ cells
F. Moghaddam , A. Oodi , M. Nikougoftar Zarif , N. Amirizadeh *
Keywords: Key words : CD133 antigen, Stem Cells, CXCR4 Receptor
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Sci J Iran Blood Transfus Organ 2015; 12(3): 277-286
 
Original  Article
 
 

Evaluation of platelet microparticles effect on
the expression of CXCR4 in umbilical cord blood
derived CD133+ cells
 
Moghaddam F.1, Oodi A.1, Nikougoftar Zarif M.1, Amirizadeh N.1
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
 
Abstract
Background and Objectives
Cord blood CD133+ cells are able to maintain long-term hematopoiesis and to differentiate to different hematopoietic lineages. CXCR4 overexpression is involved in successful transplantation of hematopoietic stem cells in the bone marrow. Platelet micro particles (PMP) contain CXCR4 markers and are able to transfer them into hematopoietic stem cells. Therefore, considering the importance of CD133+ cells as primitive HSCs, the effect of platelet micro particles on the expression levels of CXCR4 and CD34 markers in these cells was examined.
 
Materials and Methods
In this experimental study, cord blood CD133+ cells were isolated by MACS. Isolated cells were cultured into three groups one as control without adding PMP and the other two groups with PMP with concentrations of 5 and 10 μg/mL. The cells were cultured for five days in stem span medium. Expression of CXCR4 surface marker was analyzed by flow cytometery; the total cell number was counted by hemocytometer and colony forming units (CFU) were measured by colony assay.
 
Results
Fold increase of CD34+ and CXCR4+ cells in  the  control group and in the presence of 5 and 10 µg/mL of PMP were 52.6 ± 4.16, 64.2 ± 6.91, and 67.2 ± 6.76, respectively (p= 0.047). CXCR4+ cell percentage in the presence of 10 µg/mL PMP compared to control cells (63.8 ± 6.4) was 72.8 ± 4 (p= 0.049).
 
Conclusions
Exposure of CD133+ cells isolated from cord blood to PMP with 10 μg/mL concentration increased the expression of CXCR4 surface marker significantly.
 
Key words: CD133 antigen, Stem Cells, CXCR4 Receptor
 
 
 
 
 
 
Received:  20 Aug 2014
Accepted: 23 May 2015
 
 
 
 

Correspondence: Amirizadeh N., PhD of Hematology and Blood Banking. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601599; Fax : (+9821) 88601599
E-mail: n.amirizadeh@ibto.ir
Type of Study: Research | Subject: Stem cells
Published: 2015/09/2
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