Volume 12, Issue 2 (Summer 2015)                   Sci J Iran Blood Transfus Organ 2015, 12(2): 125-134 | Back to browse issues page

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Shirzad M, Heidarian E, Poorgheisari B, Amini Sarteshnizi N, Soorani Z, Beshkar P. The effect of hespertin in the induction of apoptosis and necrosis in lymphoblastic cell line Jurkat. Sci J Iran Blood Transfus Organ 2015; 12 (2) :125-134
URL: http://bloodjournal.ir/article-1-886-en.html
The effect of hespertin in the induction of apoptosis and necrosis in lymphoblastic cell line Jurkat
M. Shirzad , E. Heidarian , B. Poorgheisari , N.A. Amini Sarteshnizi , Z. Soorani , P. Beshkar *
Keywords: Key words: Leukemia ٬ Acute Lymphoblastic, hesperetin, Jurkat Cells, Apoptosis
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Original  Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2015; 12(2): 125-134
 
 

The effect of hespertin  in the induction of apoptosis
and necrosis in lymphoblastic cell line Jurkat
 
Shirzad M.1, Heidarian E.2, Poorgheisari B.1, Amini Sarteshnizi N.A.3, Soorani Z.1, Beshkar P.4,2
 
 
1Shahrekord Univercity Of Medical Sciences, Shahrekord, Iran
2Cellular & Molecular Research Center, Shahrekord Univercity Of Medical Sciences, Shahrekord, Iran
3Faculty Of Basic Science, Univercity Of Mohaghegh Ardabili, Ardabil, Iran
4Blood Transfusion Researcgh Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
Abstract
Background and Objectives
Acute lymphoblastic leukemia (ALL) is a neoplastic disorder and  a common malignancy in children. Apoptosis is a normal physiological process which occurs during the maintenance of tissue hemostasis. The defect in this process leads to the development of cancer. It can be induced by a variety of treatments, such as cytotoxic chemotherapy. Hesperetin, a flavonoid isolated from the red fruits, has been examined with regard to the inhibition of proliferation and induction of apoptosis in pancreatic cells. Hesperetin also activates NOTCH-1  and tumor suppressor in carcinoid tumor.
 
Materials and Methods
In this fundamental-applied research, the cell lines of C121 (Jurkat cell) were cultured in RPMI supplemented with 10% fetal bovine serum (FBS) and  Penicilin/streptomycin. The growth and survival of cells with various concentrations of hesperetin was evaluated by MTS kit. The amount of cell death by trypan blue staining and flow cytometry as well as by apoptosis kit (Partec) was examined.
 
Results
Lymphoblastic cell lines (C121) in exposure to different concentrations of hesperetin were affected and ΜM 200 drug concentration at 48 hours was reported as inhibitory concentration or IC50. The mode of cell death induced by hesperetin was found to be apoptosis, as judged by the morphologic alteration of the cells and by Anexin v conjugated with FITC and flow cytometric analysis.
 
Conclusions
Since hesperetin can induce apoptosis and reduce cell activity, it seems appropriate to be able to inhibit the growth of tumor cells.
 
Key words: Leukemia٬ Acute Lymphoblastic, hesperetin, Jurkat Cells, Apoptosis
 
 
 
Received:  18 Aug 2014
Accepted: 28 Feb 2015
 
 
 

Correspondence: Beshkar P., PhD Student of Hematology. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine and Cellular and Molecular Research Center, Shahrekord University of Medical Sciences.
Postal Code: 8813833435, Shahrekord, Iran. Tel: (+98381) 3335652-4; Fax: (+98381) 3334911
E-mail: beshkarpezhman@yahoo.com
Type of Study: Research | Subject: Hematology
Published: 2015/07/5
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