Volume 13, Issue 3 (Autumn 2016)
Sci J Iran Blood Transfus Organ 2016, 13(3): 243-247 |
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Razeghi M, Chatr Abnous N, Soleimani S, Kafi E. Evaluation of Bacterial contamination of platelet products by triple packs with and without sampling pouch in two different periods in Kerman Blood Center. Sci J Iran Blood Transfus Organ 2016; 13 (3) :243-247
URL: http://bloodjournal.ir/article-1-871-en.html
URL: http://bloodjournal.ir/article-1-871-en.html
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| Abstract (HTML) (6450 Views)
Evaluation of Bacterial contamination of platelet products
by triple packs with and without sampling pouch
in two different periods in Kerman Blood Center
Razeghi M.S.1, ChatrAbnous N.2, Soleimani S.2,3,4, Kafi E.2
1Sirjan Faculty of Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran
2School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
3Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
4Regional Educational Blood Transfusion Center, Kerman, Iran
Abstract
Background and Objectives
Bacterial infection following platelet transfusion is a major problem in transfusion medicine. The present study was carried out in order to evaluate the bacterial contamination of platelet concentrates collected at Kerman Regional Educational Blood Center.
Materials and Methods
In this cross-sectional study, 630 random samples of platelet transfusion in both groups (360 subjects using triple bags without sampling pouch and 270 samples using triple bags with sampling pouch) were selected. Bacterial growth of samples of platelet concentrates was studied in blood agar, EMB and thioglycollate broth after 48 hours at 37°C. SPSS 19 has been used for data analysis.
Results
Out of 630 samples of platelet concentrates studied, 5 (1.39%) were found positive for bacterial contamination all taken from the platelet samples prepared by triple bags without sampling pouch. The bacteria identified were as follows: Staph. Epidermidis (n = 3), Staph. saprophyticus (n= 2). In the platelet samples made from triple bags with sampling pouch, there was no contamination.
Conclusions
Due to advances in the collection, preparation and storage of platelet concentrates, transmitted bacterial infection caused by contaminated platelets dropped significantly. This is due to the efforts of effective quality control processes to improve the quality of blood transfusion.
Key words: Blood, Platelet Transfusion, Blood Transfusion, Blood Donors
Received: 26 Apr 2015
Accepted: 1 Jun 2016
Correspondence: Soleimani S., MSc of Immunology. School of Medicine, Kerman University of Medical Sciences & Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine and Regional Educational Blood Transfusion Center.
Postal Code: 7616859979, Kerman, Iran. Tel: (+98341) 3230712; Fax: (+98341) 3230017
E-mail: saeed.soleimanyamoli@gmail.com
Full-Text: (7956 Views)
References:
Transfusion 2001; 41(4): 477-82.
- Girgis SA, Ismail GA, Bahgat FE, Ali IK, Rashad SS, Ahmed SF. Rapid Detection of Bacterial Contamination in Platelet Concentrates, by Polymerase Chain Reaction and DNA Sequencing in Comparison to Conventional Automated Culture. In J Curr Microbiol App Sci 2014; 3(4): 38-52.
- Pietersz RN, Reesink HW, Panzer S, Oknaian S, Kuperman S, Gabriel C, et al. Bacterial contamination in platelet concentrates. Vox Sang 2014; 106(3): 256-83.
- Vedy D, Robert D, Gasparini D, Canellini G, Waldvogel S, Tissot JD and et al. Bacterial contamination of platelet concentrates: pathogen detection and inactivation methods. Hematol Rev 2009; 1(1): e5.
- Besson Faure I. Rapid screening for bacterial contamination of blood products. J Lab Med 2006; 30(2): 91-100. [Article in German]
- Brecher ME, Means N, Jere CS, Heath D, Rothenberg S, Stutzman LC.t al. Evaluation of an automated culture system for detecting bacterial contamination of platelets: an analysis with 15 contaminating organisms.
Transfusion 2001; 41(4): 477-82.
- Yomtovian RA, Palavecino EL, Dysktra AH, Downes KA, Morrissey AM, Bajaksouzian S, et al. Evolution of surveillance methods for detection of bacterial contamination of platelets in a university hospital, 1991 through 2004. Transfusion. 2006; 46(5): 719-30.
- Rahimkhani M, Alizadeh Mohammad Shir Z, Erfani Y. Microbial contamination detection methods in platelet concentrates. Sci J Iran Blood Transfus Organ 2008; 4(4): 265-74. [Article in Farsi]
- Schrezenmeier H, Walther-Wenke G, Müller TH, Weinauer F, Younis A, Holland-Letz T, et al. Bacterial contamination of platelet concentrates: results of a prospective multicenter study comparing pooled whole blood-derived platelets and apheresis platelets. Transfusion 2007; 47(4): 644-52.
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Sci J Iran Blood Transfus Organ 2016; 13(3): 243-247 |
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Short Communication
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Evaluation of Bacterial contamination of platelet products
by triple packs with and without sampling pouch
in two different periods in Kerman Blood Center
Razeghi M.S.1, ChatrAbnous N.2, Soleimani S.2,3,4, Kafi E.2
1Sirjan Faculty of Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran
2School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
3Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
4Regional Educational Blood Transfusion Center, Kerman, Iran
Abstract
Background and Objectives
Bacterial infection following platelet transfusion is a major problem in transfusion medicine. The present study was carried out in order to evaluate the bacterial contamination of platelet concentrates collected at Kerman Regional Educational Blood Center.
Materials and Methods
In this cross-sectional study, 630 random samples of platelet transfusion in both groups (360 subjects using triple bags without sampling pouch and 270 samples using triple bags with sampling pouch) were selected. Bacterial growth of samples of platelet concentrates was studied in blood agar, EMB and thioglycollate broth after 48 hours at 37°C. SPSS 19 has been used for data analysis.
Results
Out of 630 samples of platelet concentrates studied, 5 (1.39%) were found positive for bacterial contamination all taken from the platelet samples prepared by triple bags without sampling pouch. The bacteria identified were as follows: Staph. Epidermidis (n = 3), Staph. saprophyticus (n= 2). In the platelet samples made from triple bags with sampling pouch, there was no contamination.
Conclusions
Due to advances in the collection, preparation and storage of platelet concentrates, transmitted bacterial infection caused by contaminated platelets dropped significantly. This is due to the efforts of effective quality control processes to improve the quality of blood transfusion.
Key words: Blood, Platelet Transfusion, Blood Transfusion, Blood Donors
Received: 26 Apr 2015
Accepted: 1 Jun 2016
Correspondence: Soleimani S., MSc of Immunology. School of Medicine, Kerman University of Medical Sciences & Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine and Regional Educational Blood Transfusion Center.
Postal Code: 7616859979, Kerman, Iran. Tel: (+98341) 3230712; Fax: (+98341) 3230017
E-mail: saeed.soleimanyamoli@gmail.com
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