Volume 12, Issue 2 (Summer 2015)
Sci J Iran Blood Transfus Organ 2015, 12(2): 135-142 |
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Mirzaian F, Amirizadeh N, Nikougoftar Zarif M, Atarodi K, Nakhlestani M. Isolation and characterization of Placenta- MSC derived microvesicles. Sci J Iran Blood Transfus Organ 2015; 12 (2) :135-142
URL: http://bloodjournal.ir/article-1-859-en.html
URL: http://bloodjournal.ir/article-1-859-en.html
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Isolation and characterization of Placenta- MSC
derived microvesicles
Mirzaian F.1, Amirizadeh N.1, Nikougoftar Zarif M.1, Atarodi K.1, Nakhlestani M.1
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Abstract
Background and Objectives
Mesenchymal stem cells (MSCs) are the key elements of bone marrow and facilitate HSC maintenance in an in vitro co-culture system through the secretion of soluble factors and cell-cell contact. Cell-derived microvesicles (MVs) or microparticles have been described as a new mechanism of cell to cell communication.
Materials and Methods
In this experimental study, we obtained three placenta tissues from mothers with the informed consent under sterile condition. MSCs were isolated from the placentas; after several passages, MVs were obtained from MSC-culture-conditioned media by ultracentrifugation. Thje MVs concentration was determined in two samples by Bradford method; then, we characterized them by Transmission Electron microscopy and flowcytometer.
Results
At day 14 of the isolation, placenta-MSCs were passaged with more time for their growth required compared to BM-MSC. A different size range of microvesicles, within 40 to 160nm, was observed. The mean concentration was 125µg/mL (minimum 100µg/mL and maximum 150µg/mL) and they expressed CD29, CD44, CD73, and CD105 on their surface such as BM-MSC derived microvesicles.
Conclusions
Microvesicles express mesenchymal cell markers that are required for adhesion to other cells. So they can contribute to the effects of mesenchymal stem cells in various cultures such as co-culture with hematopoetic stem cells.
Key words: Cell-Derived Microparticles, Mesenchymal Stem Cells, Placenta
Received: 26 Apr 2014
Accepted: 17 Nov 2014
Correspondence: Amirizadeh N., PhD of Hematology and Blood Banking. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601599; Fax : (+9821) 88601599
E-mail: n.amirizadeh@ibto.ir
Full-Text: (2110 Views)
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Isolation and characterization of Placenta- MSC
derived microvesicles
Mirzaian F.1, Amirizadeh N.1, Nikougoftar Zarif M.1, Atarodi K.1, Nakhlestani M.1
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Abstract
Background and Objectives
Mesenchymal stem cells (MSCs) are the key elements of bone marrow and facilitate HSC maintenance in an in vitro co-culture system through the secretion of soluble factors and cell-cell contact. Cell-derived microvesicles (MVs) or microparticles have been described as a new mechanism of cell to cell communication.
Materials and Methods
In this experimental study, we obtained three placenta tissues from mothers with the informed consent under sterile condition. MSCs were isolated from the placentas; after several passages, MVs were obtained from MSC-culture-conditioned media by ultracentrifugation. Thje MVs concentration was determined in two samples by Bradford method; then, we characterized them by Transmission Electron microscopy and flowcytometer.
Results
At day 14 of the isolation, placenta-MSCs were passaged with more time for their growth required compared to BM-MSC. A different size range of microvesicles, within 40 to 160nm, was observed. The mean concentration was 125µg/mL (minimum 100µg/mL and maximum 150µg/mL) and they expressed CD29, CD44, CD73, and CD105 on their surface such as BM-MSC derived microvesicles.
Conclusions
Microvesicles express mesenchymal cell markers that are required for adhesion to other cells. So they can contribute to the effects of mesenchymal stem cells in various cultures such as co-culture with hematopoetic stem cells.
Key words: Cell-Derived Microparticles, Mesenchymal Stem Cells, Placenta
Received: 26 Apr 2014
Accepted: 17 Nov 2014
 |
Correspondence: Amirizadeh N., PhD of Hematology and Blood Banking. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601599; Fax : (+9821) 88601599
E-mail: n.amirizadeh@ibto.ir
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