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Omidkhoda A, Hedayati B, Amini Kafiabad S. The effect of whole blood storage time on quality of RBCs. Sci J Iran Blood Transfus Organ 2015; 11 (1) :56-63
URL: http://bloodjournal.ir/article-1-857-en.html
The effect of whole blood storage time on quality of RBCs
A. Omidkhoda , B. Hedayati , S. Amini Kafiabad *
Keywords: Key words: Red Blood Cells, Quality Control, Hemolysis
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References:
 
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Sci J Iran Blood Transfus Organ 2014; 11(1): 56-63  
 
Original Article
 
 

The effect of whole blood storage time on quality
of RBCs
 
Omidkhoda A.1,2, Hedayati B.1,2, Amini Kafi-Abad S.1
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Tehran Regional Educational Blood Transfusion Center, Tehran, Iran
 
 
 
Abstract
Background and Objectives
According to our national standards, whole blood units are stored at 22 ± 2°C prior to processing. Since there has been no study about the quality of RBCs prepared after the storage of whole blood units at 22 ± 2°C in Iran, we decided to investigate the quality effect of whole blood stored for 8 and 24 hours at this temperature.
 
Materials and Methods
Twelve whole blood units in pediatric bags were collected and placed in the cooling plate box. After 8 and 24 hours, RBCs were produced from these stored units. Then, the bags were analyzed for hemolysis, 2, 3-diphosphoglycerate, lactate dehydrogenase, glucose, and sodium.
 
Results
Although a higher percentage of hemolysis, lactate dehydrogenase levels, and lower 2,3-DPG, sodium and glucose levels were determined in RBCs prepared after the 24-hour WB storing time, no significant differences, except for 2,3 DPG, were observed between 8-hour (205 ± 12) and 24-hour (113 ± 13) WB storing time periods.
 
Conclusions 
Although storing whole blood at 22 ± 2°C for 24 hours prior to RBCs production had an impact on its quality, the property of prepared components is defined to be within an acceptable range of quality control.
 
Key words: Red Blood Cells, Quality Control, Hemolysis
 
 
 
 
 
 
 
 
 
Received:   7 Nov 2012
Accepted: 11 Jun 2013
 
 
 

Correspondence: Amini Kafi-Abad S., MD. Specialist in Pathology . Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14496-13111, Tehran, Iran. Tel: (+9821) 88601558; Fax: (+9821) 88601542
E-mail: s.amini@ibto.ir
Type of Study: Research | Subject: Blood transfusion medicine
Published: 2014/03/16
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