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Sci J Iran Blood Transfus Organ 2015, 11(1): 12-22 Back to browse issues page
The effect of Baicalin, a PPARy activator, on erythroid differentiation of CD133+ cord blood hematopoietic stem cells
P. Abbasi, K. Shams Asanjan, A.A. Movasaghpour, P. Akbarzadeh Laleh, N. Dehdilani
Keywords: Key words: baicalin, Erythropoiesis, Hematopoietic Stem Cells, PPAR gamma
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Type of Study: Research | Subject: Stem cells
Published: 2014/03/16
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References:
 
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Sci J Iran Blood Transfus Organ 2014; 11(1): 12-22
 
Original Article
 


 

The effect of Baicalin, a PPARy activator, on erythroid differentiation of CD133+ cord blood hematopoietic
stem cells
 
 
 
Abbasi P.1, Shams Asanjan K.2,3,4, Movasaghpour A.A.1, Akbarzadeh Laleh P.5, Dehdilani N.6
 
 
1Tabriz University of Medical Sciences, Tabriz, Iran
2Hematology Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
4Tabriz Educational Regional Blood Transfusion Center, Tabriz, Iran
5Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
6Mashhad University of Medical Sciences, Mashhad, Iran
 
Abstract
Background and Objectives
The effect of Baicalin, a PPARy activator, was investigated on erythroid differentiation of CD133+ cord blood hematopoietic stem cells.
 
Materials and Methods
The effect of PPARγ agonists (Baicalin and Troglitazone) were evaluated in erythropoiesis. CD133+ cells were isolated from human cord blood using MACS technology and cultured in the medium containing PPARγ agonists in a dose-dependent manner with SCF (20 ng/mL) and Epo (4.5 u/mL). The morphology of CD133+ cells, cell surface markers (Transferrin receptor and glycophorin A), and the formation of erythroid colony were assessed with microscopicanalysis, flow cytometry, and colony forming assay, respectively.
 
Results
Microscopic and flow cytometric analysis revealed the differentiation of CD133+ cord blood hematopoietic stem cells to erythroid lineage. Flow cytometry showed that both of the PPARγ agonists were able to diminish significantly Transferrin receptor and glycophorin A positive cell population. The inhibitory effect of PPARγ agonists on erythroid differentiation was dose-dependent. The colonies formation of erythroid was suppressed by both of PPARy agonists; hence, the effect of troglitazone was markedly higher. The inhibitory effect of Troglitazone on colonies formation of  Erythroid was also markedly higher than Bicaline.
 
Conclusions 
Our findings demonstrated that PPARy agonists modulates erythroid differentiation of CD133+ hematopoietic stem cell; therefore, they might play an important role in regulating normal erythropoiesis in vivo. The inhibitory effect of Baicalin on erythropoiesis should be noted because this herbal remedy mostly is used to treat a wide range of diseases.
 
Key words: baicalin, Erythropoiesis, Hematopoietic Stem Cells, PPAR gamma
 
Received:   6 Jan 2013
Accepted: 16 Jul 2013
 

 Correspondence: Shams Asanjan K., PhD of Hematology and Blood Banking. Assistant Professor of Hematology Oncology Research Center, Tabriz University of Medical Sciences and Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine and Tabriz Educational Regional Blood Transfusion Center.
P.O.Box: 51335, Tabriz , Iran. Tel: (+98411) 2871515; Fax: (+98411) 2871515
                E-mail: k.shams@ibto.ir
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Abbasi P, Shams Asanjan K, Movasaghpour A, Akbarzadeh Laleh P, Dehdilani N. The effect of Baicalin, a PPARy activator, on erythroid differentiation of CD133+ cord blood hematopoietic stem cells. Sci J Iran Blood Transfus Organ. 2015; 11 (1) :12-22
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Volume 11, Issue 1 (Spring 2014 2015) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
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