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mohammadi P, soleimani S, Sharifi Z, Nouruzi A, Najafi M. Association between G-436 A (rs2333227) polymorphism at myeloperoxidase gene promoter andcoronary artery disease risk in Iranian patients. Sci J Iran Blood Transfus Organ 2015; 11 (4) :286-294
URL: http://bloodjournal.ir/article-1-838-en.html
Association between G-436 A (rs2333227) polymorphism at myeloperoxidase gene promoter andcoronary artery disease risk in Iranian patients
P. Mohammadi , S. Soleimani , Z. Sharifi , A. Nouruzi , M. Najafi *
Keywords: Key words: Coronary Artery Disease, Reactive Oxygen Species, Coronary Stenosis, Risk Factors
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Original  Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2015; 11(4): 286-294
 
 
 
 
 


Association between G-436 A (rs2333227) polymorphism at myeloperoxidase gene promoter and coronary artery
disease risk in Iranian patients
 
Mohammadi P.1, Soleimani S.2, Sharifi Z.2, Nouruzi A.3, Najafi M.4
 
  1. Tehran University of Medical Sciences, Tehran, Iran
  2. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran 
  3. Tehran University of Medical Sciences, Tehran, Iran
  4. Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran
 
 
Background and Objectives
Polymorphonuclear cell-derived myeloperoxidase (MPO) is a known agent in the progression of inflammatory disorders via the production of reactive oxygen species. In this study, we investigated the association between rs2333227 polymorphism (G-436 A) within the MPO gene promoter and coronary artery stenosis.
 
Materials and Methods
A total of 160 Iranian subjects (patients = 86 and controls = 74) were introduced on the basis of the study criteria. DNA was extracted from white blood cells and the genotype distribution was evaluated by PCR-RFLP technique
 
Results
The AA, AG and GG genotype frequencies in study population were calculated to be 6.90%, 39.4% and 53.7%, respectively (p > 0.21). There was no significant difference between the genotype distribution and the extent of coronary stenosis in patients with 1VD, 2VD and 3VD (p = 0.71). The A allele frequencies were 25.6% and 27.4% in patients and controls, respectively.
 
Conclusions
In general, our results indicate that the Iranian population genotype and allele distribution do not play a role in the development and severity of coronary artery disease.
 
Key words: Coronary Artery Disease, Reactive Oxygen Species, Coronary Stenosis, Risk Factors
 
 
 
 
 
 
 
Received: 22 Jan 2014
Accepted:14 Jun 2014
 
 
 

Correspondence: Najafi M., PhD of Biochemistry. Associate Professor of Immunology Research Center, Iran University of Medical Sciences.
P.O.Box: 14155-5983, Tehran, Iran. Tel: (+9821) 86703107; Fax: (+9821) 86703107
E-mail:
nbsmmsbn@tums.ac.ir
Type of Study: Research | Subject: Biochemistry
Published: 2014/12/31
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