Volume 10, Issue 4 (winter 2014)                   Sci J Iran Blood Transfus Organ 2014, 10(4): 319-325 | Back to browse issues page

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Shiri R, Yari F, Amirizadeh N, Gharehbaghian A, Ahmadinejad M, Tabatabaie M. Effects of caspase 3 inhibitor on the survival of platelet concentrate during storage . Sci J Iran Blood Transfus Organ 2014; 10 (4) :319-325
URL: http://bloodjournal.ir/article-1-837-en.html
Effects of caspase 3 inhibitor on the survival of platelet concentrate during storage
R. Shiri , F. Yari * , N. Amirizadeh , A. Gharehbaghian , M. Ahmadinejad , M.R. Tabatabaie
Keywords: Key words: Platelets, Caspase 3, Caspase Inhibitors
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Original Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2014; 10(4): 319-325
 
 
 
 

Effects of caspase 3 inhibitor on the survival of platelet concentrate during storage
 
Shiri R.1, Yari F.1, Amirizadeh N.1, Gharehbaghian A.2,3, Ahmadinejad M.1, Tabatabaie M.R.1
 
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Paramedical Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Pediatric Congenital Hematologic Disorders Research Center of Shahid Beheshti University of Medical Sciences,Tehran, Iran
 
 
Abstract
Background and Objectives
The time for platelet concentrate storage is 3 to 5 days. One of the limitations for platelet storage was storage lesion in which they lose their viability and undergo apoptosis. Studies show that apoptosis occurs in some anucleated cells such as platelets. In this study, the caspase 3 inhibitor was employed in platelet concentrates to obtain platelets with higher survival. 
 
Materials and Methods
After preparation of platelet concentrates, caspase3 inhibitor (16 μM) was injected to the test bags. These bags along with control bags which lacked the inhibitor were stored at 22  in shaker incubator for 7 days. Samples from these bags were obtained and the activity of caspase3 and the survival levels of platelets were analyzed. 
 
Results
In the presence of caspase3 inhibitor, the activity of caspase3 in platelets decreased compared to the control group. Additionally, this study showed that the survival of platelets decreased during storage but in the presence of caspase3 inhibitor the survival of platelet concentrate was significantly higher than that of the control group (p<0.05).     
 
Conclusions 
Injection of caspase3 inhibitor to the platelet concentrate can reduce apoptosis and therefore increase their survival.
 
Key words: Platelets, Caspase 3, Caspase Inhibitors
 
 
 
 
 
 
Received: 18 Aug  2012
Accepted: 14 Aug 2013
 
 
 
 

Correspondence: Yari F., PhD of Immunology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
          P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052237; Fax: (+9821) 88601555
E-mail: f.yari@ibto.ir
Type of Study: Research | Subject: Hematology
Published: 2013/12/29
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