Volume 10, Issue 4 (winter 2014)                   Sci J Iran Blood Transfus Organ 2014, 10(4): 364-371 | Back to browse issues page

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Nikoozad R, Mahzounieh M, Naghavi N. Genomic detection of parvovirus B19 in patients suffered from chronic anemia, leukemia/lymphoma in Isfahan, Iran. Sci J Iran Blood Transfus Organ 2014; 10 (4) :364-371
URL: http://bloodjournal.ir/article-1-828-en.html
Genomic detection of parvovirus B19 in patients suffered from chronic anemia, leukemia/lymphoma in Isfahan, Iran
R. Nikoozad * , M.R. Mahzounieh , N.S. Naghavi
Keywords: Key words: Parvovirus, Hematologic Diseases, PCR, Iran
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Original Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2014; 10(4): 364-371
 
 

Genomic detection of parvovirus B19 in patients
suffered from chronic anemia, leukemia/lymphoma
in Isfahan, Iran
 
Nikoozad R.1, Mahzounieh M.R.2, Naghavi N.S.1
 
 
1Faculty of Biological Science, Islamic Azad University Falavarjan Branch, Falavarjan, Iran
2Zoonosis Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
 
 
Abstract
Background and Objectives
Parvovirus B19 may cause a number of clinical illnesses including infectious erythem, arthropathy hydropes fetalis or congenital anemia, and transient aplastic crises. The virus is very infectious and is transmitted via respiratory tract, and blood transfusion. The aim of this study was to detect B19 virus in persons who suffer from blood disorders.
 
Materials and Methods
Parvovirus B19 infection was compared in patients suffering from blood disorders with healthy people in Isfahan, Iran. Sera were collected from 35 and 30 patients with the history of chronic anemia and lukemia/lymphoma. Thirty persons were considered as control group without any blood complications. DNAs of all sera were extracted and tested for presence of B19 DNA using semi-nested PCR.
 
Results
B19 DNA was detected in 15 samples (23.1%) out of 65 patient sera. The infection rate was 17.1% and 30% among benign and malignant blood disorder groups, respectively. None of healthy people showed positive results.
 
Conclusions 
Results are consistent with previous studies indicating that parvovirus B19 infection in high-risk individuals is higher including patients with hematologic disorders. Blood donor screening for parvovirus B19 in high risk patients is necessary and can effectively reduce the incidence and prevalence of B19 infection.
 
Key words: Parvovirus, Hematologic Diseases, PCR, Iraniseases, PCR, Isfahanic d, Iran.
 
 
 
 
 
 
Received:  6 Feb 2013
Accepted:18 Jun 2013
 
 
 
 

Correspondence: Nikoozad R., MSc of Microbiology. Faculty of Biological Science, Islamic Azad University Falavarjan Branch.
P.O.Box: 84515-155, Falavarjan, Iran. Tel: (+98311) 7420140; Fax: (+98311) 7420140
E-mail: R_nikoozad@yahoo.com
Type of Study: Research | Subject: Hematology
Published: 2013/12/21
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