Volume 11, Issue 4 (winter 2015)                   Sci J Iran Blood Transfus Organ 2015, 11(4): 295-305 | Back to browse issues page

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Heydari-keshel S, Rezaei Taviranii M, Ai J, Soleimani M, Ghanbari Z, Baradaran-Rafii A. Isolation and characterization of endometrial mesenchymal stem cells and the evaluation of surface markers in comparison to bone marrow mesenchymal stem cells. Sci J Iran Blood Transfus Organ 2015; 11 (4) :295-305
URL: http://bloodjournal.ir/article-1-804-en.html
Isolation and characterization of endometrial mesenchymal stem cells and the evaluation of surface markers in comparison to bone marrow mesenchymal stem cells
S. Heydari-keshel * , M. Rezaei Taviranii , J. Ai , M. Soleimani , Z. Ghanbari , A.R. Baradaran-Rafii
Proteomics Research Center, Faculty of paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Keywords: Key words : Endometrium, Adult Stem Cells, Flow Cytometry
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Sci J Iran Blood Transfus Organ 2015; 11(4): 295-305
 
Original  Article
 

 

Isolation and characterization of endometrial
mesenchymal stem cells and the evaluation of surface
markers in comparison to bone marrow
mesenchymal stem cells
 
Heidari-Keshel S.1, Rezaei Tavirany M.1, Ai J.2, Soleimani M.3, Ghanbari Z.4,
Baradaran-Rafii A.R.5
 
1Proteomics Research Center, Faculty of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Faculty of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran
3Tarbiat Modares University, Tehran, Iran
4Tehran University of Medical Sciences, Tehran, Iran.
5Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
 
Abstract
Background and Objectives
Stem cells are undifferentiated cells able to self-renew as well as to produce more differentiated daughter cells. In this study, stem cells were isolated from the endometrium and were then compared with the mesenchymal stem cells for their nature to be determined.
 
Materials and Methods
Endometrial samples from healthy donors with informed consent after laparoscopy were collected by a gynecologist. The bone marrow samples were aspirated from femur by a hematologist. At least 5 mL of blood was removed and the tissue is at least one square centimeter. After three successive passages, the stem cell surface markers of CD90, CD105, CD146, CD34, CD73, CD44, CD133, and CD45 were analyzed using flowcytometry. The karyotype analysis was also performed for the adherent cells.
 
Results
Endometrial stem cells were successfully isolated from the five samples. Adherent cells in flowcytometric analysis showed the positive expression of surface markers to be CD146: 59.3% , CD105: 98.3%, CD90: 95.8%, CD34: 0.9%, CD 45: 1.1%, CD73: 96%, CD44: 84%, and CD133: 1.4%  while in bone marrow mesenchymal stem cells the expression of markers were CD105: 98.6%, CD90: 98.4%, CD34: 0.5%, CD73: 97.9%, CD44: 89.3%, CD146:13%, CD45: 0.3%, and CD133: 0.6%. The morphology of endometrial cells, spindle-like mesenchymal cells, the mode of application, and 44XX karyotype were normal.
 
Conclusions
Stem cells isolated from the endometrial tissue had a heterogeneous population of the cells and high levels of expression of specific surface markers of stem cells especially the mesoderm lineage.
 
Key words: Endometrium, Adult Stem Cells, Flow Cytometry
 
Received:   3 Nov 2013
Accepted: 17 Jun 2014
 
 

 

Correspondence: Rezaei Tavirany M., Protemics Research Center, Faculty of paramedical Sciences, Shahid Beheshti University of Medical Sciences.
Postal Code: 1971653313, Tehran, Iran. Tel: (+9821) 22714248; Fax: (+9821) 22714248
E-mail: tavirany@yahoo.com
Type of Study: Research | Subject: Stem cells
Published: 2014/12/31
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