Volume 10, Issue 3 (Autumn 2013)                   Sci J Iran Blood Transfus Organ 2013, 10(3): 222-230 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Oubari F, Nikougoftar Zarif M, Amirizadeh N, Shaiegan M, Atarodi K, Nakhlestani M, et al . Isolation and expansion of Mesenchymal Stem Cells from placenta. Sci J Iran Blood Transfus Organ 2013; 10 (3) :222-230
URL: http://bloodjournal.ir/article-1-791-en.html
Isolation and expansion of Mesenchymal Stem Cells from placenta
F. Oubari , M. Nikougoftar Zarif * , N. Amirizadeh , M. Shaiegan , K. Atarodi , M. Nakhlestani , M. Mirzamoradi , R. Fadai , Kh. Golzade
Keywords: Mesenchymal Stem Cells, Placenta, Cell Therapy
Full-Text [PDF 579 kb]   (3992 Downloads)     |   Abstract (HTML)  (12511 Views)
Full-Text:   (3893 Views)
References:
  1. Mafi R, Hindocha S, Mafi P, Griffin M, Khan WS. Sources of adult mesenchymal stem cells applicable for musculoskeletal applications-a systematic review of the literature. Open Orthop J 2011; 5 Suppl 2: 242-8.
  2. Celebi B, Mantovani D, Pineault N. Irradiated Mesenchymal Stem Cells improve Irradiated the ex vivo expansion of Hematopoietic progenitor by party mimicking the bone marrow endosteal environment. J Immunol Methods 2011; 370(1-2): 93-103.
  3. Jing D, Fonseca AV, Alakel N, Fierro FA, Muller K, Bornhauser M, et al. Hematopoietic stem cells in co-culture with mesenchymal stromal cells--modeling the niche compartments in vitro. Haematologica 2010; 95(4): 542-50.
  4. Lee OK, Kuo TK, Chen WM, Lee KD, Hsieh SL, Chen TH. Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood 2004; 103(5): 1669-75.
  5. Vater C, Kasten P, Stiehler M. Culture media for the differentiation of mesenchymal stromal cells. Acta Biomater 2011; 7(2): 463-77.
  6. Li CD, Zhang WY, Li HL, Jiang XX, Zhang Y, Tang PH, et al. Mesenchymal stem cells derived from human placenta suppress allogeneic umbilical cord blood lymphocyte proliferation. Cell Res 2005; 15(7): 539-47.
  7. Bowman TV, Zon LI. Lessons from the Niche for Generation and Expansion of Hematopoietic Stem Cells. Drug Discov Today Ther Strateg 2009; 6(4): 135-40.
  8. Andrade-Zaldívar H, Santos L, De León Rodríguez A. Expansion of human hematopoietic stem cells for transplantation:        trends         and           perspectives.
 
 
 
Cytotechnology 2008; 56(3): 151-60.
  1. Tan J, Liu T, Hou L, Meng W, Wang Y, Zhi W, et al. Maintenance and expansion of hematopoietic stem/progenitor cells in biomimetic osteoblast niche. Cytotechnology 2010; 62(5): 439-48.
  2. Walenda T, Bokermann G, Ventura Ferreira MS, Piroth DM, Hieronymus T, Neuss S, et al. Synergistic effects of growth factors and mesenchymal stromal cells for expansion of hematopoietic stem and progenitor cells. Exp Hematol 2011; 39(6): 617-28.
  3. Phinney DG, Prockop DJ. Mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair--current views. Stem Cells 2007; 25(11): 2896-902.
  4. Battula VL, Treml S, Abele H, Bühring HJ. Prospective isolation and characterization of mesenchymal stem cells from human placenta using a frizzled-9-specific monoclonal antibody. Differentiation 2008; 76(4): 326-36.
  5. Kanematsu D, Shofuda T, Yamamoto A, Ban C, Ueda T, Yamasaki M, et al. Isolation and cellular properties of mesenchymal cells derived from the decidua of human term placenta. Differentiation 2011; 82(2): 77-88.
  6. Miao Z, Jin J, Chen L, Zhu J, Huang W, Zhao J, et al. Isolation of mesenchymal stem cells from human placenta: comparison with human bone marrow mesenchymal stem cells. Cell Biol Int 2006; 30(9): 681-7.
  7. Barlow S, Brooke G, Chatterjee K, Price G, Pelekanos R, Rossetti T, et al. Comparison of human placenta-and bone marrow-derived multipotent mesenchymal stem cells. Stem Cells Dev 2008; 17(6): 1095-107.
 
 

 
 
 
Original Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2013; 10(3): 222-230
 
 
 
 

Isolation and expansion of Mesenchymal Stem
 Cells from placenta
 
Oubari F.1, Nikougoftar Zarif M.1, Amirizadeh N.1, Shaiegan M.1, Atarodi K.1, Nakhlestani M.1,
Mirzamoradi M.2,3, Fadai R.1, Golzade Kh.1
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Mahdieh Educational Hospital, Tehran, Iran
 
 
Abstract
Background and Objectives
Mesenchymal stem cells are fibroblasts with the capacity of proliferation, colonogenesis, and differentiation to the mesodermal cells that are known as the source for cell therapy purposes. These cells are present in some adult and embryonic tissues in which the bone marrow serves as the main source of human mesenchymal stem cells. Because of some limitations in using the bone marrow, in this study we established a method for isolation of mesenchymal stem cells from placenta and characterized their properties.
 
Materials and Methods
In this experimental study, we obtained 3 placenta tissues from informed consent mothers after normal vaginal delivery. Mesenchymal stem cells were isolated from the placenta, cultured, and were then examined for their proliferation, colonogenesis, immunophenotyping and differentiation capacities from passages 3 and 7.
 
Results
Our results showed the high capacity of proliferation and colonogenesis of placental derived mesenchymal stem cells. Immunophenotyping confirmed more than 95% purity of isolated cells; their surface antigen expression showed the phenotypical properties like those of bone marrow derived mesenchymal stem cells. The cells had the osteocytic and adipocytic differentiation capacity.
 
Conclusions 
In this study we successfully isolated mesenchymal stem cells from placenta and cultured them without any alteration in their capacities; consequently, we found out that we can isolate and expand these cells as an alternative source for cell therapies.
 
Key words: Mesenchymal Stem Cells, Placenta, Cell Therapy
 
 
Received: 29 May 2012
Accepted:30 Dec  2012
 
 
 

Correspondence: Nikougoftar Zarif M., PhD of Hematology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601575; Fax: (+9821) 88601576
E-mail: Nikougoftar@ibto.ir
Type of Study: Research | Subject: Stem cells
Published: 2013/10/7
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Scientific Journal of Iran Blood Transfus Organ

Designed & Developed by : Yektaweb