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Nouri M, Ebrahini M, Baghsheykhi A, Akhlaghi A, Mohammad M, Samimi S et al . The evaluation of hematopoietic stem cells potential after 2 years of cryopreservation. Sci J Iran Blood Transfus Organ 2015; 12 (1) :1-12
URL: http://bloodjournal.ir/article-1-758-en.html
The evaluation of hematopoietic stem cells potential after 2 years of cryopreservation
M. Nouri , M. Ebrahini * , A.H. Baghsheykhi , A. Akhlaghi , M. Mohammad , S. Samimi , M. Zarabi
Keywords: Key words: Hematopoietic Stem Cells, Cryopreservation, Colony-Forming Units Assay
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Sci J Iran Blood Transfus Organ 2015; 12(1): 1-12
 
Original  Article
 


 


The evaluation of hematopoietic stem cells potential
after 2 years of cryopreservation
 
Nouri M.1, Ebrahimi M1, Baghsheykhi A.H.1, Akhlaghi A.2, Mohammad M.1,
Samimi S.3, Zarabi M.3
 
 
1Department of Stem Cells and Development Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
2Royan Institute for Stem Cell Biology and Technology, Tehran, Iran 
3Royan Stem Cell Technology Company, Tehran, Iran
 
 
 
 
Abstract
Background and Objectives
The evaluation of the number, potential, and functional capacity of cord blood stem cells after long term storage is essential for their therapeutic uses in hematological malignancies and non-malignancies. Therefore, this study was aimed to assess the quality of cord blood units stored in Royan public cord blood bank, before and after cryopreservation.
 
Materials and Methods
To check the quality of cryopreserverd cord blood units, the total number of cells, percentage of viable cells, colony-forming ability, the number of CD34+ cells before and after freezing were examined on 50 samples
 
Results
Our results determined that the mean value of the total nucleated cells in 50 cord blood samples was 7.6 × 108 ± 1.12 × 108. The number and viability of mononuclear cells as well as colony forming potential significantly decreased after cryopreservation; however, the count of viable CD34+ cells did not significant changes post thawing. There was no significant correlation between the numbers of nucleated cells and CD34+ cells.
 
Conclusions 
Increasing the storage time significantly reduces the number of nucleated cells and differentiation potential of hematopoietic stem cells rather than the number of CD34+ cells. Improved cryopreservation techniques are necessary to maintain the function of cord blood stem cells.
 
Key words: Hematopoietic Stem Cells, Cryopreservation, Colony-Forming Units Assay
 
 
 
 
Received: 25 May 2013
Accepted:  6 Aug  2014
 
 
 

Correspondence: Ebrahimi M., PhD of Immunology. Associate Professor of Department of Regenerative Biomedicine at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR.
P.O.Box: 19395-4644, Tehran, Iran. Tel: (+9821) 23562000; Fax: (+9821) 22413790
E-mail:
mebrahimi@royaninstitute.org
Type of Study: Research | Subject: Blood banking
Published: 2015/04/7
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