Volume 10, Issue 1 (Spring 2013)                   Sci J Iran Blood Transfus Organ 2013, 10(1): 40-52 | Back to browse issues page

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Hosseini A, Halabian R, Hamedi Asl P, Bashiri Nahanji H, Jalili M, Heydari M, et al . Role of autophagy as a survival factor in MSCs following exposure to oxidative stress . Sci J Iran Blood Transfus Organ 2013; 10 (1) :40-52
URL: http://bloodjournal.ir/article-1-738-en.html
Role of autophagy as a survival factor in MSCs following exposure to oxidative stress
A. Hosseini , R. Halabian , P. Hamedi Asl , H. Bashiri Nahanji , M.A. Jalili , M. Heydari , N. Amirizadeh , M. Habibi Roudkenar *
Keywords: Key words: Mesenchymal Stem Cells, Autophagy, Oxidative Stress, Cell Hypoxia, Rapamycin
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Original Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2013; 10 (1): 40-52
 
 
 

Role of autophagy as a survival factor in MSCs following  exposure  to oxidative stress
 
 
Hosseini A.1, Halabian R.2, Hamedi Asl P.1, Bashiri Nahanji H.1, Jalili M.A.1,  Heydari M.1,
 Amiri zade N. 1, Habibi Roudkenar M.1
 
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran,  Iran
2Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
 
 
 
Abstract
Background and Objectives
Previous studies have showed that a large percentage of Mesenchymal Stem Cells (MSCs) die in the early stages of transplantation due to oxidative streeses, hypoxia, and serum deprivation. Hence, this study was aimed to address whether induction or inhibition of autophagy would affect the viability of MSCs after exposure to oxidative stress.
 
Materials and Methods
In this descriptive study, MSCs were isolated from bone marrow with ficol and density gradient method; the fourth passage MSCs were selected in the study. Autophagy was detected by using transfection of GFP-LC3 to MSCs. Induction and inhibition of autophagy were performed by using rapamycin and 3MA, respectively. MSCs were exposed to lethal doses of H2O2 followed by cells viability evaluated with MTT assay.
 
Results
Our results revealed that the enhancement of autophagy in MSCs sensitized them against oxidative stress and inhibition of autophagy led to resistance against oxidative stress in comparison with the control cells.
 
Conclusions 
Inhibition of autophagy using non genetic engineering method in MSCs enhances cell viability following exposure to the oxidative stress. This may provide a novel strategy to promote the efficiency of cell therapies following transplantation in the future.
 
Key words: Mesenchymal Stem Cells, Autophagy, Oxidative Stress, Cell Hypoxia, Rapamycin
 
 
 
Received:    7 May 2012
Accepted: 23 Sep   2012
 
 
 

Correspondence: Habibi Roudkenar M., PhD of Biotechnology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
                P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601599; Fax: (+9821) 88601599
                E-mail: roudkenar@ibto.ir
Type of Study: Research | Subject: Stem cells
Published: 2013/08/14
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