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:: Volume 20, Issue 4 (Winter 2023) ::
Sci J Iran Blood Transfus Organ 2023, 20(4): 267-275 Back to browse issues page
Association of HLA-DR polymorphisms and Febrile Non-Hemolytic Transfusion Reaction in multi-transfused thalassemia patients
Z. Tasbiti , M.S. Dadashi , A. Chegini , M. Zadsar *
Keywords: Febrile Non-Hemolytic Transfusion Reaction, Blood Transfusion, Polymorphism (Genetics), HLA-DR, Thalassemia
Full-Text [PDF 426 kb]   (457 Downloads)     |   Abstract (HTML)  (1080 Views)
Type of Study: Research | Subject: Blood transfusion medicine
Published: 2023/12/31
Full-Text:   (238 Views)



Sci J Iran Blood Transfus Organ 2023;20 (4): 267-275
Original Article
 
Association of HLA-DR polymorphisms and Febrile
Non-Hemolytic Transfusion Reaction in multi-transfused
thalassemia patients

Tasbiti Z.1, Dadashi M.S.1, Chegini A.1, Zadsar M.1


1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


Abstract
Background and Objectives
β-thalassemia patients need regular blood. Regular blood transfusion has side effects. The most common of which is FNHTR. One of the causes of FNHTR is the antibody production against HLA-II antigens. HLA polymorphism vary in different communities and may affect the susceptibility or resistance to FNHTR. In this study, the relationship between HLA-DR polymorphisms and development of FNHTR in multi-transfused thalassemia patients in Tehran province was investigated.

Materials and Methods
In this cross-sectional study, 88 β-thalassemia major and intermediate patients with average age 38.63 ± 11.28 with 35 being male (39.8%) and 53 female (60.2%) were enrolled and for better results, 70 thalassemia patients with FNHTR were compared with 18 thalassemia patients without FNHTR. HLA-DR genotype was investigated by PCR-SSP method. Data were analyzed by chi-squared and pearson test.

Results
The results of PCR-SSP showed that HLA-DRB1*11 allele had the highest prevalence (26.7%) in the total study population. No statistically significant correlation between HLA-DR alleles and development of FNHTR was observed. Although HLA-DRB1*10 as a possible genetic marker may play a role in protecting against FNHTR (p = 0.049).

Conclusions 
In this study, no significant association between HLA-DR allele frequency and FNHTR was found. Although alleles HLA-DRB1*03, HLA-DRB1*10, HLA-DRB1*13 and HLA-DRB1*16 have higher prevalence in the no FNHTR group, but only in the case of HLA-DRB1*10 this relationship was significant and was considered as possible genetic markers against development of FNHTR. Further studies investigating HLA-II allelic levels in larger populations may provide more deep data on this association.

Key words: Febrile Non-Hemolytic Transfusion Reaction, Blood Transfusion, Polymorphism (Genetics), HLA-DR, Thalassemia



Received:  9 Oct 2023
Accepted: 4 Nov 2023



Correspondence: Zadsar M., MD. Specialist in Infectious Diseases. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88621248; Fax: (+9821) 88601555
E-mail: maryam.zad@gmail.com
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Tasbiti Z, Dadashi M, Chegini A, Zadsar M. Association of HLA-DR polymorphisms and Febrile Non-Hemolytic Transfusion Reaction in multi-transfused thalassemia patients. Sci J Iran Blood Transfus Organ 2023; 20 (4) :267-275
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Volume 20, Issue 4 (Winter 2023) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
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